SSX2 Overexpression Lysate (Native)

Images

 
Western Blot: SSX2 Overexpression Lysate (Adult Normal) [NBL1-16484] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for SSX2.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB

Order Details

SSX2 Overexpression Lysate (Native) Summary

Immunogen
The lysate was created in HEK293T cells, using plasmid ID RC203504 and based on accession number NM_175698. The protein contains a C-terminal DDK tag.
Specificity
Homo sapiens synovial sarcoma, X breakpoint 2 (SSX2), transcript variant 2, mRNA.
Gene
SSX2

Applications/Dilutions

Application Notes
This product is intended for use as a positive control in Western Blot. You will receive the lysate (100ug), and an empty vector negative control (100 ug).
Theoretical MW
21.4 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -80C. Avoid freeze-thaw cycles.
Buffer
RIPA buffer

Lysate Details for SSX2

Type
Overexpression
Protein State
Native

Notes

HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.This product is manufactured by and sold under license from OriGene Technologies and its use is limited solely for research purposes.

Alternate Names for SSX2 Overexpression Lysate (Native)

  • Cancer/testis antigen 5.2
  • cancer/testis antigen family 5, member 2a
  • CT5.2a
  • HD21
  • HOM-MEL-40
  • MGC119055
  • MGC15364
  • MGC3884
  • protein SSX2
  • sarcoma, synovial, X-chromosome-related 2
  • SSX
  • SSX2A
  • SSX2B
  • synovial sarcoma, X breakpoint 2B
  • synovial sarcoma, X breakpoint 2CT5.2
  • Tumor antigen HOM-MEL-40

Background

The product of this gene belongs to the family of highly homologous synovial sarcoma X (SSX) breakpoint proteins. These proteins may function as transcriptional repressors. They are also capable of eliciting spontaneously humoral and cellular immune responses in cancer patients, and are potentially useful targets in cancer vaccine-based immunotherapy. SSX1, SSX2 and SSX4 genes have been involved in the t(X;18) translocation characteristically found in all synovial sarcomas. This translocation results in the fusion of the synovial sarcoma translocation gene on chromosome 18 to one of the SSX genes on chromosome X. The encoded hybrid proteins are probably responsible for transforming activity. Two transcript variants encoding distinct isoforms have been identified for this gene. [provided by RefSeq]. Transcript Variant: This variant (2) lacks a segment in the coding region, which leads to a frameshift, compared to variant 1. The resulting isoform (b) contains a shorter and distinct C-terminus compared to isoform a. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

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Publications for SSX2 Lysate (NBL1-16484) (0)

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Bioinformatics

Gene Symbol SSX2