SSH2 Overexpression Lysate (Denatured)

Images

 
Lane 1: SSH2 transfected lysate (19.69 KDa) Lane 2: Non-transfected lysate.
SSH2 transfected lysate.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB

Order Details

SSH2 Overexpression Lysate (Denatured) Summary

Description
Pasmid: pCMV-SSH2 full-length
Gene
SSH2

Packaging, Storage & Formulations

Storage
Store at -80C. Avoid freeze-thaw cycles.
Buffer
Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, and 0.01% Bromophenol blue).

Lysate Details for SSH2

Type
Overexpression
Protein State
Denatured

Notes

Quality control test: Transient overexpression cell lysate was tested with Anti-SSH2 antibody by Western Blots.

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for SSH2 Overexpression Lysate (Denatured)

  • EC 3.1.3.16
  • EC 3.1.3.48
  • hSSH-2L
  • KIAA1725protein phosphatase Slingshot homolog 2
  • MGC78588
  • slingshot 2
  • slingshot homolog 2 (Drosophila)
  • SSH-2
  • SSH2L
  • SSH-2L
  • SSH-like protein 2

Background

The ADF (actin-depolymerizing factor)/cofilin family (see MIM 601442) is composed of stimulus-responsive mediators of actin dynamics. ADF/cofilin proteins are inactivated by kinases such as LIM domain kinase-1 (LIMK1; MIM 601329). The SSH family appears to play a role in actin dynamics by reactivating ADF/cofilin proteins in vivo (Niwa et al., 2002 [PubMed 11832213]).[supplied by OMIM]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

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Publications for SSH2 Lysate (H00085464-T01) (0)

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Product General Protocols

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Video Protocols

WB Video Protocol

FAQs for SSH2 Lysate (H00085464-T01). (Showing 1 - 1 of 1 FAQ).

  1. Can this product, SSH2 overexpressed cell lysate (H00085464-T01), be directly used to screen the small molecule inhibitors for SSH2 in enzymatic reaction?
    • H00085464-T01 is produced by a Taiwanese company called Abnova We do no in-house testing or development of their products. This product is a lysate of SSH2 overexpressed in 293T cells. The buffer is 50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue so the protein will not be active, so is most likely not suitable for your screen.

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Bioinformatics

Gene Symbol SSH2