SSH2 293T Cell Transient Overexpression Lysate

Lane 1: SSH2 transfected lysate (19.69 KDa) Lane 2: Non-transfected lysate.

SSH2 transfected lysate.

• Reactivity: Hu
• Applications: WB

SSH2 293T Cell Transient Overexpression Lysate Summary

• Description: Plasmid: pCMV-SSH2 full-length
• Specificity: SSH2 293T Cell Transient Overexpression Lysate(Denatured)
• Gene: SSH2

Applications/Dilutions

• Dilutions:
  • Western Blot

Packaging, Storage & Formulations

• Storage: Store at -80C. Avoid freeze-thaw cycles.
• Buffer: 1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue)

Lysate Details for Array

• Type: 293T Cell Transient Overexpression
• Protein State: Denatured

Notes

Quality control test: Transient overexpression cell lysate was tested with Anti-SSH2 antibody by Western Blots.

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for SSH2 293T Cell Transient Overexpression Lysate

• EC 3.1.3.16
• EC 3.1.3.48
• hSSH-2L
• KIAA1725protein phosphatase Slingshot homolog 2
• MGC78588
• slingshot 2
• slingshot homolog 2 (Drosophila)
• SSH-2
• SSH2L
• SSH-2L
• SSH-like protein 2

Background

The ADF (actin-depolymerizing factor)/cofilin family (see MIM 601442) is composed of stimulus-responsive mediators of actin dynamics. ADF/cofilin proteins are inactivated by kinases such as LIM domain kinase-1 (LIMK1; MIM 601329). The SSH family appears to play a role in actin dynamics by reactivating ADF/cofilin proteins in vivo (Niwa et al., 2002 [PubMed 11832213]).[supplied by OMIM]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

FAQs for SSH2 Lysate (H00085464-T01). (Showing 1 - 1 of 1 FAQ).

1. Can this product, SSH2 overexpressed cell lysate (H00085464-T01), be directly used to screen the small molecule inhibitors for SSH2 in enzymatic reaction?
   • H00085464-T01 is produced by a Taiwanese company called Abnova We do no in-house testing or development of their products. This product is a lysate of SSH2 overexpressed in 293T cells. The buffer is 50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue so the protein will not be active, so is most likely not suitable for your screen.

Bioinformatics

• Gene Symbol: SSH2