SRY Overexpression Lysate (Native)

Images

 
Western Blot: SRY Overexpression Lysate (Adult Normal) [NBL1-16462] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for SRY.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB

Order Details

SRY Overexpression Lysate (Native) Summary

Immunogen
The lysate was created in HEK293T cells, using plasmid ID RC210382 and based on accession number NM_003140. The protein contains a C-terminal DDK tag.
Specificity
Homo sapiens sex determining region Y (SRY), mRNA.
Gene
SRY

Applications/Dilutions

Application Notes
This product is intended for use as a positive control in Western Blot. You will receive the lysate (100ug), and an empty vector negative control (100 ug).
Theoretical MW
23.7 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -80C. Avoid freeze-thaw cycles.
Buffer
RIPA buffer

Lysate Details for SRY

Type
Overexpression
Protein State
Native

Notes

HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.This product is manufactured by and sold under license from OriGene Technologies and its use is limited solely for research purposes.

Alternate Names for SRY Overexpression Lysate (Native)

  • essential protein for sex determination in human males
  • sex determining region Y
  • sex-determining region on Y
  • sex-determining region Y protein
  • TDFsex determining region protein
  • TDY
  • Testis-determining factor

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

Customers Who Viewed This Item Also Viewed...

AF3075
Species: Hu
Applications: WB, ICC
PP-N1665-00
Species: Hu
Applications: WB, IHC, IP
NBP1-80583
Species: Hu
Applications: IHC, IHC-P
PP-H7431-00
Species: Hu
Applications: WB
NBP1-19371
Species: Hu, Mu, Rt, Ca, Rb
Applications: WB, Simple Western, ICC/IF, IHC, IHC-P
NB110-60011
Species: Hu, Mu
Applications: WB, Simple Western, IHC, IHC-Fr, IHC-P, IP
NBP2-41198
Species: Hu, Mu
Applications: WB, ELISA
NB100-56603
Species: Hu, Pm
Applications: WB, IHC, IHC-P
AF475
Species: Mu
Applications: WB, IHC
H00007536-M01
Species: Hu, Mu
Applications: WB, ELISA, ICC/IF, IHC-P, IP
22240002
Species: Hu
Applications: WB, ELISA
NBP1-91642
Species: Hu
Applications: ICC/IF, IHC, IHC-P
MAB2018
Species: Hu, Mu, Rt
Applications: WB, Simple Western, CyTOF-reported, ICC, ICFlow
NBP2-20383
Species: Hu, Mu
Applications: WB, ICC/IF, IHC, IHC-P
H00001617-M01
Species: Hu
Applications: WB, ELISA, ICC/IF, IHC-P
AF2569
Species: Hu
Applications: WB, ICC
NBP1-32887
Species: Hu
Applications: WB, IHC, IHC-P
NBL1-16462
Species: Hu
Applications: WB

Publications for SRY Lysate (NBL1-16462) (0)

There are no publications for SRY Lysate (NBL1-16462).
By submitting your publication information earn gift cards and discounts for future purchases.

Reviews for SRY Lysate (NBL1-16462) (0)

There are no reviews for SRY Lysate (NBL1-16462). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen
  • 1st to review with an image -- $50/€35/£30/$50 CAD/¥300 Yuan/¥5000 Yen

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for SRY Lysate (NBL1-16462) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Customers Who Bought This Also Bought

CD4 Antibody
NBP1-19371

Contact Information

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our SRY Overexpression Lysate (Native) and receive a gift card or discount.

Bioinformatics

Gene Symbol SRY