SRPRB Antibody - BSA Free

Western Blot: SRPRB Antibody [NBP2-66584] - Western blot analysis using SRPRB antibody on 3 (1), 6 (2) and 12 (3) ng of canine microsomal protein.

• Reactivity: Ca, Ch
• Applications: WB, IHC
• Clonality: Polyclonal
• Host: Sheep
• Conjugate: Unconjugated
• Format: BSA Free

SRPRB Antibody - BSA Free Summary

• Description: Novus Biologicals Sheep SRPRB Antibody - BSA Free (NBP2-66584) is a polyclonal antibody validated for use in IHC and WB. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Synthetic peptide corresponding to amino acids 246-265 derived from the carboxyl terminal of the canine SRPRB subunit.
• Specificity: Antibody will not detect SRPRB protein bound to SRPRA
• Isotype: IgG
• Clonality: Polyclonal
• Host: Sheep
• Gene: SRPRB
• Purity: Ammonium sulfate precipitation

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:10 - 1:500
  • Western Blot 15 ug/ml
• Application Notes: .

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.08% Sodium Azide
• Purity: Ammonium sulfate precipitation

Alternate Names for SRPRB Antibody - BSA Free

• APMCF1
• Protein APMCF1
• signal recognition particle receptor subunit beta
• signal recognition particle receptor, B subunit
• signal recognition particle receptor, beta subunit
• SR-beta

Background

The receptor for Signal Recognition Particle (SRP) is the site on the endoplasmic reticulum that ribosomes translating secreted and integral membrane proteins are initially targeted to. Once the ribosome-SRP complex arrives at the SRP receptor the protein being translated is transferred to the translocation complex (Sec61) in the ER membrane. The SRP receptor is composed of two subunits SRalpha and SRbeta. The SRalpha subunit is a translocation GTPase peripherally bound to the endoplasmic reticulum by its interaction with SRbeta. SRalpha also binds to the GTPase of SRP (SRP54) and these two proteins appear to function as each others GTPase activating proteins (GAPs). Hydrolysis of GTP by SRalpha and SRP54 is thought to be involved in transfer of the nascent protein to the Sec61 complex in the ER. SRalpha has an apparent molecular weight of 72 kDa. SRbeta is a Type I transmembrane protein that spans the membrane once and contains Ras type GTPase domain. The function of the GTPase in SRbeta is unknown. The membrane spanning domain is at the amino-terminus of SRbeta. The GTPase domain encompasses three quarters of the protein and is carboxyl- of the transmembrane region. SRalpha binds to the GTPase domain of SRbeta. Heterodimerization of SRalpha and SRbeta masks the carboxyl-terminal epitope of SRbeta.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: SRPRB