SRGAP1 Antibody

Western Blot: SRGAP1 Antibody [H00057522-B01P] - Analysis of SRGAP1 expression in transfected 293T cell line by SRGAP1 polyclonal antibody. Lane 1: SRGAP1 transfected lysate(119.35 KDa). Lane 2: Non-transfected lysate.

Immunocytochemistry/Immunofluorescence: SRGAP1 Antibody [H00057522-B01P] - Analysis of purified antibody to SRGAP1 on HeLa cell. (antibody concentration 10 ug/ml)

Product Discontinued

SRGAP1 Antibody Summary

• Immunogen: SRGAP1 (NP_065813.1, 1 a.a. - 1085 a.a.) full-length human protein. MSTPSRFKKDKEIIAEYESQVKEIRAQLVEQQKCLEQQTEMRVQLLQDLQDFFRKKAEIETEYSRNLEKLAERFMAKTRSTKDHQQYKKDQNLLSPVNCWYLLLNQVRRESKDHATLSDIYLNNVIMRFMQISEDSTRMFKKSKEIAFQLHEDLMKVLNELYTVMKTYHMYHAESISAESKLKEAEKQEEKQIGRSGDPVFHIRLEERHQRRSSVKKIEKMKEKRQAKYSENKLKSIKARNEYLLTLEATNASVFKYYIHDLSDLIDCCDLGYHASLNRALRTYLSAEYNLETSRHEGLDIIENAVDNLEPRSDKQRFMEMYPAAFCPPMKFEFQSHMGDEVCQVSAQQPVQAELMLRYQQLQSRLATLKIENEEVKKTTEATLQTIQDMVTIEDYDVSECFQHSRSTESVKSTVSETYLSKPSIAKRRANQQETEQFYFMKLREYLEGSNLITKLQAKHDLLQRTLGEGHRAEYMTTRPPNVPPKPQKHRKSRPRSQYNTKLFNGDLETFVKDSGQVIPLIVESCIRFINLYGLQHQGIFRVSGSQVEVNDIKNSFERGENPLADDQSNHDINSVAGVLKLYFRGLENPLFPKERFNDLISCIRIDNLYERALHIRKLLLTLPRSVLIVMRYLFAFLNHLSQYSDENMMDPYNLAICFGPTLMPVPEIQDQVSCQAHVNEIIKTIIIHHETIFPDAKELDGPVYEKCMAGDDYCDSPYSEHGTLEEVDQDAGTEPHTSEDECEPIEAIAKFDYVGRSARELSFKKGASLLLYHRASEDWWEGRHNGIDGLVPHQYIVVQDMDDTFSDTLSQKADSEASSGPVTEDKSSSKDMNSPTDRHPDGYLARQRKRGEPPPPVRRPGRTSDGHCPLHPPHALSNSSVDLGSPSLASHPRGLLQNRGLNNDSPERRRRPGHGSLTNISRHDSLKKIDSPPIRRSTSSGQYTGFNDHKPLDPETIAQDIEETMNTALNELRELERQSTAKHAPDVVLDTLEQVKNSPTPATSTESLSPLHNVALRSSEPQIRRSTSSSSDTMSTFKPMVAPRMGVQLKPPALRPKPAVLPKTNPTIGPAPPPQGPTDKSCTM
• Specificity: SRGAP1 - SLIT-ROBO Rho GTPase activating protein 1,
• Isotype: IgG
• Clonality: Polyclonal
• Host: Mouse
• Gene: SRGAP1
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence
  • Western Blot 1:500
• Application Notes: Antibody reactive against Recombinant Protein with GST tag on ELISA and Western Blot and also on transfected lysate in western blot. GST tag alone is used as a negative control. Has also been used for immunofluorescence.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.4)
• Preservative: No Preservative
• Purity: Protein A purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for SRGAP1 Antibody

• ARHGAP13srGAP1
• FLJ22166
• KIAA1304SLIT-ROBO Rho GTPase-activating protein 1
• Rho GTPase-activating protein 13
• SLIT-ROBO Rho GTPase activating protein 1

Background

GTPase-activating protein for RhoA and Cdc42 small GTPases. Together with CDC42 seems to be involved in thepathway mediating the repulsive signaling of Robo and Slit proteins in neuronal migration. SLIT2, probably throughinteraction with ROBO1, increases the interaction of SRGAP1 with ROBO1 and inactivates CDC42

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: SRGAP1
• Entrez:
  • 57522(Human)
• Uniprot:
  • NP_065813.1(Human)