Src Antibody (JF0947)

Western Blot: Src Antibody (JF0947) [NBP2-67167] - Analysis of Src on NIH/3T3 cell lysates with Rabbit anti-Src antibody at 1/500 dilution. Lysates/proteins at 10 ug/Lane. Predicted band size: 60 kDa Observed band size: 60 kDa Exposure time: 2 minutes; 8% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:300,000 dilution was used for 1 hour at room temperature.

Immunocytochemistry/Immunofluorescence: Src Antibody (JF0947) [NBP2-67167] - Staining Src in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunohistochemistry-Paraffin: Src Antibody (JF0947) [NBP2-67167] - Analysis of paraffin-embedded rat brain tissue using anti-Src antibody. Counter stained with hematoxylin.

Flow Cytometry: Src Antibody (JF0947) [NBP2-67167] - Analysis of NIH/3T3 cells with Src antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody

Western Blot: Src Antibody (JF0947) [NBP2-67167] - Analysis of Src on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: HUVEC cell lysateLane 2: A549 cell lysateLane 3: PC-3M cell lysateLane 4: SH-SY5Y cell lysate

Immunocytochemistry/Immunofluorescence: Src Antibody (JF0947) [NBP2-67167] - Staining Src in A431 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunocytochemistry/Immunofluorescence: Src Antibody (JF0947) [NBP2-67167] - Staining Src in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunohistochemistry-Paraffin: Src Antibody (JF0947) [NBP2-67167] - Analysis of paraffin-embedded human breast carcinoma tissue using anti-Src antibody. Counter stained with hematoxylin.

Immunohistochemistry-Paraffin: Src Antibody (JF0947) [NBP2-67167] - Analysis of paraffin-embedded human kidney tissue using anti-Src antibody. Counter stained with hematoxylin.

Immunohistochemistry-Paraffin: Src Antibody (JF0947) [NBP2-67167] - Analysis of paraffin-embedded human tonsil tissue using anti-Src antibody. Counter stained with hematoxylin.

Immunohistochemistry-Paraffin: Src Antibody (JF0947) [NBP2-67167] - Analysis of paraffin-embedded mouse kidney tissue using anti-Src antibody. Counter stained with hematoxylin.

Product Discontinued

Src Antibody (JF0947) Summary

• Additional Information: Recombinant Monoclonal Antibody.
• Immunogen: Synthetic peptide within human Src aa 20-60. (SwissProt: P12931 Human; SwissProt: P05480 Mouse; SwissProt: Q9WUD9 Rat)
• Localization: Cell membrane, Mitochondrion inner membrane, Nucleus, Cytoplasm.
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: SRC
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 1:50-1:100
  • Immunocytochemistry/ Immunofluorescence 1:100-1:500
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1:50-1:200
  • Western Blot 1:1000-1:5000

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: TBS (pH7.4), 0.05% BSA, 40% Glycerol
• Preservative: 0.05% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein A purified

Alternate Names for Src Antibody (JF0947)

• ASV
• c-Src
• EC 2.7.10
• EC 2.7.10.2
• pp60c-src
• Rous sarcoma
• RSVgp4
• Src
• tyrosine kinase pp60c-src
• tyrosine-protein kinase SRC-1
• v-src avian sarcoma (Schmidt-Ruppin A-2) viral oncogene homolog
• v-src sarcoma (Schmidt-Ruppin A-2) viral oncogene homolog (avian)

Background

Src is a protein tyrosine kinase known to regulate cellular adhesion. Composed of three major domains; SH2, SH3 and a kinase catalytic domain, Src can be switched from an inactive to an active state though control of its phosphorylation state or through protein-protein interaction. Phosphorylation of Y529 by Csk and Chk inactivates Src, while dephosphorylation of Y529 will modify its conformation to an open active state (1-3). Mutation of Y529 leads to Scr overactivity. Several cancers including colon and breast cancer have been associated with an increase of Src activity (4).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: SRC