SPIRE1 Antibody (4C5) - Azide and BSA Free

Western Blot: SPIRE1 Antibody (4C5) [H00056907-M01] - Analysis of SPIRE1 expression in transfected 293T cell line by SPIRE1 monoclonal antibody (M01), clone 4C5.Lane 1: SPIRE1 transfected lysate(15 KDa).Lane 2: Non-transfected lysate.

Sandwich ELISA: SPIRE1 Antibody (4C5) [H00056907-M01] - Detection limit for recombinant GST tagged SPIRE1 is approximately 0.03ng/ml as a capture antibody.

• Reactivity: Hu, Ha
• Applications: WB, ELISA
• Clone: 4C5
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free

SPIRE1 Antibody (4C5) - Azide and BSA Free Summary

• Immunogen: SPIRE1 (NP_064533, 482 a.a. ~ 583 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. SEKPSTAHHRPLRSIARFSSKSKSMDKSDEELQFPKELMEDWSTMEVCVDCKKFISEIISSSRRSLVLANKRARLKRKTQSFYMSSPGPSEYCPSERTISEI
• Specificity: SPIRE1 - spire homolog 1 (Drosophila)
• Isotype: IgG2a Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: SPIRE1
• Purity: IgG purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Sandwich ELISA
  • Western Blot 1:500
• Application Notes: Antibody reactive against recombinant protein with GST tag on ELISA and Western Blot. GST tag alone is used as a negative control.

Reactivity Notes

Human. Other species not tested.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: In 1x PBS, pH 7.4
• Preservative: No Preservative
• Purity: IgG purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for SPIRE1 Antibody (4C5) - Azide and BSA Free

• KIAA1135
• MGC150621
• MGC150622
• protein spire homolog 1
• SPIR1
• Spir-1
• spire homolog 1 (Drosophila)

Background

Spire proteins, such as SPIRE1, are highly conserved between species. They belong to the family of Wiskott-Aldrich homology region-2 (WH2) proteins, which are involved in actin organization (Kerkhoff et al., 2001 [PubMed 11747823]).[supplied by OMIM]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for SPIRE1 Antibody (H00056907-M01)(3)

Publications using H00056907-M01

• Luo Y, Barrios-Rodiles M, Gupta GD Atypical function of a centrosomal module in WNT signalling drives contextual cancer cell motility Nat Commun 2019-05-29 [PMID: 31142743] (Human)
• Morel E, Parton RG, Gruenberg J et al. Annexin A2-Dependent Polymerization of Actin Mediates Endosome Biogenesis . Dev Cell;16(3):445-57. 2009-03-01 [PMID: 19289089]
• Thom SR, Bhopale VM, Mancini DJ, Milovanova TN. Actin S-Nitrosylation Inhibits Neutrophil {beta}2 Integrin Function. J Biol Chem. 2008-04-18 [PMID: 18283105]

Bioinformatics

• Gene Symbol: SPIRE1
• Entrez:
  • 56907(Human)
• OMIM:
  • 609216(Human)
• Uniprot:
  • NP_064533(Human)