Spinesin Antibody

Images

 
Spinesin was detected in perfusion fixed frozen sections of mouse brain (cerebellum) using Goat Anti-Mouse Spinesin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1928) at 15 µg/mL overnight at ...read more

Product Details

Summary
Reactivity MuSpecies Glossary
Applications WB, IHC, IP
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
View Available Formulations
Catalog# & Formulation Size Price

Spinesin Antibody Summary

Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse Spinesin
Tyr61-Arg445
Accession # NP_109634
Specificity
Detects mouse Spinesin in direct ELISAs and Western blots. In direct ELISAs and Western blots, approximately 20% cross-reactivity with recombinant human Spinesin is observed.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
TMPRSS5
Purity
Immunogen affinity purified
Purity Statement
Antigen Affinity-purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 0.1 ug/mL
  • Immunohistochemistry 5-15 ug/mL
  • Immunoprecipitation 25 ug/mL

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Purity
Immunogen affinity purified
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Spinesin Antibody

  • EC 3.4.21
  • EC 3.4.21.-
  • EC 3.4.21.4
  • MGC141886
  • MGC148044
  • Spinesin
  • TMPRSS5
  • transmembrane protease serine 5
  • transmembrane protease, serine 5

Background

Spinesin, encoded by the TMPRSS5 gene, is a new member of type II transmembrane serine proteases (TTSPs) (1). Mouse Spinesin contains the following structural domains: a short N-terminal cytoplasmic tail, a transmembrane domain, a stem region and a serine protease domain (2). The domain structure of Spinesin is common to other TTSPs, many of which have additional domains. The stem region of Spinesin contains a scavenger receptor-like domain. There could be 4 types of transcripts due to alternative splicing (3). Type 4 predicts 10 extra amino acids at the N-terminus as compared to type 3. The ectodomain corresponding to type 3 (residues 61‑445) or  type 4 (residues 71‑455) was expressed and purified as a single chain pro-enzyme. By SDS-PAGE, the pro-enzyme migrates as multiple forms, possibly due to differential glycosylation. The pro-enzyme can be activated by trypsin treatment. The resulting enzyme is active and its activity is measured as described above. The activated enzyme is a disulfide bond-linked dimer.

  1. Shibata, K. et al. (2000) Genome Res. 10:1757.
  2. Yamaguchi, Y. et al. (2002) J. Biol. Chem. 277:6806.
  3. Watanable, Y. et al. (2004) Biochem. Biophys. Res. Commun. 324:333.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customers Who Viewed This Item Also Viewed...

4776-SE
AF2209
Species: Hu
Applications: WB, ELISA, IHC
NBP1-03232
Species: Hu, Mu, Rt
Applications: WB, ELISA, ICC/IF, IHC, IHC-P
NBP1-20984
Species: Hu
Applications: WB, Simple Western, ICC/IF, IHC, IHC-P, PEP-ELISA
NB100-77342
Species: Hu
Applications: WB, PEP-ELISA
NBP1-56991
Species: Hu
Applications: WB
AF3946
Species: Hu
Applications: WB, CyTOF-ready, ICFlow
NBP2-67279
Species: Hu, Mu, Rt
Applications: WB, ELISA, ICC/IF, IHC, IHC-P
MAB2695
Species: Hu
Applications: WB
NBP2-67584
Species: Hu, Mu, Rt
Applications: WB, Flow, ICC/IF, IHC, IHC-P, IP
H00008458-M06
Species: Hu, Mu, Rt
Applications: WB, ELISA, ICC/IF
NBP1-33485
Species: Hu, Mu, Po
Applications: WB, ICC/IF, IHC, IHC-P
NBP1-58268
Species: Hu, Mu
Applications: WB, IHC, IHC-P

Publications for Spinesin Antibody (AF1928) (0)

There are no publications for Spinesin Antibody (AF1928).
By submitting your publication information earn gift cards and discounts for future purchases.

Reviews for Spinesin Antibody (AF1928) (0)

There are no reviews for Spinesin Antibody (AF1928). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for Spinesin Antibody (AF1928) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Biotin BAF1928

Additional Spinesin Products

Bioinformatics Tool for Spinesin Antibody (AF1928)

Discover related pathways, diseases and genes to Spinesin Antibody (AF1928). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for Spinesin Antibody (AF1928)

Discover more about diseases related to Spinesin Antibody (AF1928).
 

Pathways for Spinesin Antibody (AF1928)

View related products by pathway.

PTMs for Spinesin Antibody (AF1928)

Learn more about PTMs related to Spinesin Antibody (AF1928).

Blogs on Spinesin

There are no specific blogs for Spinesin, but you can read our latest blog posts.
Coronavirus Brochure

Customers Who Bought This Also Bought

Contact Information

Learn the difference between western blot and simple western

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our Spinesin Antibody and receive a gift card or discount.

Bioinformatics

Gene Symbol TMPRSS5
Uniprot