Spinesin Antibody (282324) [Alexa Fluor® 750]

• Reactivity: Mu
• Applications: WB, IP
• Clone: 282324
• Clonality: Monoclonal
• Host: Rat
• Conjugate: Alexa Fluor 750

Spinesin Antibody (282324) [Alexa Fluor® 750] Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant mouse Spinesin
  Tyr61-Arg445
  Accession # NP_109634
• Specificity: Detects mouse Spinesin in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross-reactivity with recombinant human Spinesin is observed.
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Rat
• Purity: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • Immunoprecipitation
  • Western Blot

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
• Buffer: Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide
• Purity: Protein A or G purified

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Spinesin Antibody (282324) [Alexa Fluor® 750]

• EC 3.4.21
• EC 3.4.21.-
• EC 3.4.21.4
• MGC141886
• MGC148044
• Spinesin
• TMPRSS5
• transmembrane protease serine 5
• transmembrane protease, serine 5

Background

Spinesin, encoded by the TMPRSS5 gene, is a new member of type II transmembrane serine proteases (TTSPs) (1). Mouse Spinesin contains the following structural domains: a short N-terminal cytoplasmic tail, a transmembrane domain, a stem region and a serine protease domain (2). The domain structure of Spinesin is common to other TTSPs, many of which have additional domains. The stem region of Spinesin contains a scavenger receptor-like domain. There could be 4 types of transcripts due to alternative splicing (3). Type 4 predicts 10 extra amino acids (aa) at the N-terminus as compared to type 3. The ectodomain corresponding to type 3 (aa 61‑445) or  type 4 (aa 71‑455) was expressed and purified as a single chain pro-enzyme. By SDS-PAGE, the pro-enzyme migrates as multiple forms, possibly due to differential glycosylation. The pro-enzyme can be activated by trypsin treatment. The resulting enzyme is active and its activity is measured as described above. The activated enzyme is a disulfide bond-linked dimer.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.