SPATA32 Overexpression Lysate Summary
| Description |
Transient overexpression lysate of chromosome 17 open reading frame 46 (C17orf46) The lysate was created in HEK293T cells, using Plasmid ID RC207055 and based on accession number NM_152343. The protein contains a C-MYC/DDK Tag. |
| Gene |
SPATA32 |
Applications/Dilutions
| Application Notes |
This product is intended for use as a positive control in Western Blot.
You will receive 1 vial of lysate (100ug), 1 vial of empty vector negative control (100ug), and 1 vial of 2xSDS sample buffer (250ul). |
| Theoretical MW |
42.1 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
Packaging, Storage & Formulations
| Storage |
Store at -80C. Avoid freeze-thaw cycles. |
| Buffer |
RIPA buffer |
Lysate Details for Array
Notes
HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.This product is manufactured by and sold under license from OriGene Technologies and its use is limited solely for research purposes.
Alternate Names for SPATA32 Overexpression Lysate
Limitations
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are
guaranteed for 6 months from date of receipt.
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Blogs on SPATA32
![Western Blot: C17orf46 Overexpression Lysate (Adult Normal) [NBP2-05388] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for C17orf46.](http://images.novusbio.com/fullsize/C17orf46 Overexpression Lysate (Adult Normal)-Western Blot-NBP2-05388-img0001.jpg "Western Blot: C17orf46 Overexpression Lysate (Adult Normal) [NBP2-05388] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for C17orf46.")
![Western Blot: beta-Actin Antibody (AC-15) [NB600-501] - MCDK cells induced with C22H24N2O8 to control the expression of the gene of interest. Beta-actin blocking confirms the albumin assay showing that an equal amount of lysate was loaded in each lane.](http://images.novusbio.com/images2/beta-Actin-Antibody-(AC-15)-Western-Blot-NB600-501-img0023.jpg)
![Simple Western: beta-Actin Antibody (AC-15) [NB600-501] - Analysis using the HRP conjugate of NB600-501. Image shows a specific band for Beta-Actin in 0.2 mg/mL of Hela, MCF-7, SH-SY5Y, & Jurkat lysate(s). This experiment was performed under reducing conditions using the 12-230 kDa separation system. * Non-specific interaction with the 230 kDa Simple Western standard may be seen with this antibody.](http://images.novusbio.com/images2/beta-Actin-Antibody-(AC-15)-Simple-Western-NB600-501-img0028.jpg)
![Western Blot: beta-Actin Antibody (AC-15) [NB600-501] - Beta-Actin expression in Fibroblast Hs68 cell lysate using anti-beta-Actin antibody. Image from verified customer review.](http://images.novusbio.com/images2/beta-Actin-Antibody-(AC-15)-Western-Blot-NB600-501-img0026.jpg)
![Western Blot: GALM Antibody [NBP2-38252] - Lane 1: Marker [kDa] 250, 130, 100, 70, 55, 35, 25, 15, 10Lane 2: Human Kidney tissue](http://images.novusbio.com/images2/GALM-Antibody-Western-Blot-NBP2-38252-img0003.jpg)
![Immunocytochemistry/Immunofluorescence: GALM Antibody [NBP2-38252] - Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm.](http://images.novusbio.com/images2/GALM-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-38252-img0004.jpg)
![Immunohistochemistry-Paraffin: GALM Antibody [NBP2-38252] - Staining of human cerebral cortex.](http://images.novusbio.com/images2/GALM-Antibody-Immunohistochemistry-Paraffin-NBP2-38252-img0009.jpg)
![SDS-Page: Recombinant Human ATP5G3 Protein [H00000518-P01] - 12.5% SDS-PAGE Stained with Coomassie Blue.](http://images.novusbio.com/images2/Recombinant-Human-ATP5G3-Protein-SDS-Page-H00000518-P01-img0001.jpg)
![Immunocytochemistry/Immunofluorescence: MHC Class I Antibody (OX18) [NB120-6405] - PC12 cells were fixed for 10 minutes using 4% PFA and then permeabilized for 5 minutes using 1X PBS + 0.05% Triton-X100. The cells were incubated with anti-MHC Class I Antibody (OX18) at 2 ug/ml overnight at 4C and detected with an anti-mouse Dylight 488 (Green) at a 1:500 dilution. Actin was detected with Phalloidin 568 (Red) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.](http://images.novusbio.com/images2/MHC-Class-I-Antibody-(OX18)-Immunocytochemistry-Immunofluorescence-NB120-6405-img0010.jpg)
![Immunohistochemistry-Paraffin: MHC Class I Antibody (OX18) [NB120-6405] - Analysis of FFPE rat brain cerebellum using MHC Class I (OK18) antibody at 1:200 on a Bond Rx autostainer (Leica Biosystems). The assay involved 20 minutes of heat induced antigen retrieval (HIER) using 10mM sodium citrate buffer (pH 6.0) and endogenous peroxidase quenching with peroxide block. The sections were incubated with primary antibody for 30 minutes and Bond Polymer Refine Detection (Leica Biosystems) with DAB was used for signal development followed by counterstaining with hematoxylin. Whole slide scanning and capturing of representative images was performed using Aperio AT2 (Leica Biosystems). Endothelial staining was observed. Staining was performed by Histowiz.](http://images.novusbio.com/images2/MHC-Class-I-Antibody-(OX18)-Immunohistochemistry-Paraffin-NB120-6405-img0008.jpg)
![Flow Cytometry: MHC Class I Antibody (OX18) [NB120-6405] - Analysis using the FITC conjugate of NB120-6405. Staining of rat spleen cells with mouse anti-rat RT1-A (OX18).](http://images.novusbio.com/images2/MHC-Class-I-Antibody-(OX18)-Flow-Cytometry-NB120-6405-img0007.jpg)
![Western Blot: Hsp47 Antibody (M16.10A1) [NBP1-97491] - analysis of Hsp47 in NIH/3T3 cell lysate (20ug/lane) using anti-Hsp47 antibody. 5x10^4 NIH/3T3 cells were seeded in 6-well plate and transfected with 50nM of HSP47 siRNA the following day. Total protein was harvested 72h after transfection. Image from verified customer review.](http://images.novusbio.com/images2/Hsp47-Antibody-(M16.10A1)-Western-Blot-NBP1-97491-img0006.jpg)
![Immunohistochemistry-Frozen: Hsp47 Antibody (M16.10A1) [NBP1-97491] - Hsp47 antibody in guinea pig skin (subdermis). Image from verified customer review.](http://images.novusbio.com/images2/Hsp47-Antibody-(M16.10A1)-Immunohistochemistry-Frozen-NBP1-97491-img0004.jpg)
![Western Blot: Hsp47 Antibody (M16.10A1) [NBP1-97491] - Analysis of HSP47, mAb (M16.10A1): Lane 1: Rat recombinant HSP47; Lane 2: Rat-2 (heat shocked); Lane 3: L929 (heat shocked); Lane 4: 3T3 (heat shocked); Lane 5: CHO (heat shocked); Lane 6: HeLa (heat shocked).](http://images.novusbio.com/images/Hsp47-Antibody-(M16.10A1)-Western-Blot-NBP1-97491-img0003.jpg)
-img0001.jpg)
![Western Blot: beta-Galactosidase-1/GLB1 Antibody (OTI1C9) [NBP2-45731] - Analysis of extracts (35ug) from 9 different cell lines by using anti-GLB1 monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).](http://images.novusbio.com/images2/beta-Galactosidase-1-GLB1-Antibody-(1C9)-Western-Blot-NBP2-45731-img0016.jpg)
![Immunohistochemistry: beta-Galactosidase-1/GLB1 Antibody (1C9) [NBP2-45731] - Analysis of Adenocarcinoma of Human ovary tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)](http://images.novusbio.com/images2/beta-Galactosidase-1-GLB1-Antibody-(1C9)-Immunohistochemistry-NBP2-45731-img0013.jpg)
![Western Blot: beta-Galactosidase-1/GLB1 Antibody (OTI1C9) [NBP2-45731] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY GLB1.](http://images.novusbio.com/images2/beta-Galactosidase-1-GLB1-Antibody-(1C9)-Western-Blot-NBP2-45731-img0014.jpg)
![Western Blot: csl/RBPJK Antibody [NBP1-33427] - Sample (30 ug of whole cell lysate) A: Jurkat 7.5% SDS PAGE diluted at 1:2000](http://images.novusbio.com/images2/csl-RBPJK-Antibody-Western-Blot-NBP1-33427-img0007.jpg)
![Immunocytochemistry/Immunofluorescence: csl/RBPJK Antibody [NBP1-33427] - Analysis of paraformaldehyde-fixed HeLa at 1:100 dilution.](http://images.novusbio.com/images2/csl-RBPJK-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-33427-img0004.jpg)
![Chromatin Immunoprecipitation (ChIP): csl/RBPJK Antibody [NBP1-33427] - Sheared, cross-linked chromatin was prepared from Jurkat T-ALL cells using the Covaris TruChIP kit and shearing with the LE220 sonicator. The chromatin buffer was adjusted to 150 mM NaCl, 20 mM Tris-Cl pH 8, 2 mM EDTA, and 1% Triton X-100. Three antibodies, as well as pure pre-immune rabbit IgG control, were added to each IP reaction, consisting of 7 million cells, and incubated overnight with mixing at 4 C. The antibodies were captured with Magna ChIP Protein A+G beads (Millipore), washed with three wash buffers, and the DNA eluted and purified with the iPure v2 kit (Diagenode). The purified DNA was normalized to the input control with qPCR quantification of the insulin exon 1 gene (INS) and an RBPJ binding site in the promoter of HES1. Image from verified customer review.](http://images.novusbio.com/images2/csl-RBPJK-Antibody-Chromatin-Immunoprecipitation-(ChIP)-NBP1-33427-img0006.jpg)