SPAM1 Synthetic Peptide

• Reactivity: Hu
• Applications: WB, AC
• Concentration: LYOPH

SPAM1 Synthetic Peptide Summary

• Description: 55kDa synthetic peptide located within the following region: QQQNVQLSLTEATEKAKQEFEKAGKDFLVETIKLGKLLRPNHLWGYYLFP (Uniprot: P38567)
  
  The peptide is characterized by mass spectroscopy
• Source: Synthetic
• Protein/Peptide Type: Synthetic Peptide
• Gene: SPAM1
• Purity: Multi-step

Applications/Dilutions

• Dilutions:
  • Antibody Competition
  • Western Blot
• Application Notes: This peptide is useful as a blocking peptide for NBP2-83580. It may block above mentioned antibody from binding to its target protein in western blot and/or immunohistochemistry under proper experimental settings.
• Theoretical MW: 55 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Concentration: LYOPH
• Purity: Multi-step
• Reconstitution Instructions: Add 100ul of sterile PBS. Final peptide concentration is 1 mg/ml in PBS

Alternate Names for SPAM1 Synthetic Peptide

• EC 3.2.1.35
• HYA1
• HYAL1
• HYAL3
• HYAL5
• hyal-PH20
• hyaluronidase PH-20
• Hyaluronoglucosaminidase PH-20
• MGC26532
• PH20
• PH-20
• SPAG15
• SPAM1
• sperm adhesion molecule 1 (PH-20 hyaluronidase, zona pellucida binding)
• Sperm adhesion molecule 1
• Sperm surface protein PH-20

Background

Hyaluronidase degrades hyaluronic acid, a major structural proteoglycan found in extracellular matrices and basement membranes. Six members of the hyaluronidase family are clustered into two tightly linked groups on chromosome 3p21.3 and 7q31.3. This gene was previously referred to as HYAL1 and HYA1 and has since been assigned the official symbol SPAM1; another family member on chromosome 3p21.3 has been assigned HYAL1. This gene encodes a GPI-anchored enzyme located on the human sperm surface and inner acrosomal membrane. This multifunctional protein is a hyaluronidase that enables sperm to penetrate through the hyaluronic acid-rich cumulus cell layer surrounding the oocyte, a receptor that plays a role in hyaluronic acid induced cell signaling, and a receptor that is involved in sperm-zona pellucida adhesion. Abnormal expression of this gene in tumors has implicated this protein in degradation of basement membranes leading to tumor invasion and metastasis. Multiple protein isoforms are encoded by transcript variants of this gene. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

Bioinformatics

• Gene Symbol: SPAM1