SPAM1 Antibody (763056)

Western blot shows Recombinant Bovine SPAM1 (Catalog # 6436-GH) (5 ng/lane). PVDF membrane was probed with 2 µg/mL of Mouse Anti-Bovine SPAM1 Monoclonal Antibody (Catalog # MAB6436) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for SPAM1 at approximately 78 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Product Discontinued

SPAM1 Antibody (763056) Summary

• Immunogen: Chinese hamster ovary cell line CHO-derived recombinant bovine SPAM1
  Met1-Thr497
  Accession # AAI10184
• Specificity: Detects bovine SPAM1 in direct ELISAs.
• Source: N/A
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: SPAM1
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Western Blot 2 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Sterile PBS to a final concentration of 0.5 mg/mL.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for SPAM1 Antibody (763056)

• EC 3.2.1.35
• HYA1
• HYAL1
• HYAL3
• HYAL5
• hyal-PH20
• hyaluronidase PH-20
• Hyaluronoglucosaminidase PH-20
• MGC26532
• PH20
• PH-20
• SPAG15
• SPAM1
• sperm adhesion molecule 1 (PH-20 hyaluronidase, zona pellucida binding)
• Sperm adhesion molecule 1
• Sperm surface protein PH-20

Background

Sperm adhesion molecule 1 (SPAM1), also known as PH-20 hyaluronidase (1), is encoded by one of the six hyaluronidase-like genes (1-3). SPAM1 is a GPI‑anchored enzyme located on the sperm surface and inner acrosomal membrane (4). SPAM1 degrades hyaluronic acid (HA), a major structural glycosaminoglycan found in extracellular matrices and basement membranes. The enzyme activity enables sperm to penetrate through the HA-rich cumulus cell layer surrounding the oocyte and therefore facilitates the fertilization process (5-7). Because of the structural similarity between HA (repeating units of GlcA beta 1-3GlcNAc) and chondroitin sulfate (repeating units of GlcA beta 1-3GalNAc), the enzyme is also able to hydrolyze chondroitin sulfate. The bovine SPAM1 is 63% identical to human homologue in sequence.

1. Lathrop, W.F. et al. (1990) J. Cell Biol. 111:2939.
2. Lin, Y. et al. (1993) Proc. Natl. Acad. Sci. U.S.A. 90:10071
3. Csoka, A.B. et al. (2001) Matrix Biol. 20:499.
4. Phelps, B.M. et al (1988) Science 240:4860
5. Gmachl, M. et al. (1993) FEBS Lett. 336:545.
6. Hofinger, E.S et al. (2007) Glycobiology 17:963.
7. Wu, Z.L. et al. (2010) BMC Biotechnol. 10:11. 8.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: SPAM1
• Uniprot:
  • AAI10184()