SPAG9 Overexpression Lysate

Western Blot: SPAG9 Overexpression Lysate (Adult Normal) [NBP2-07933] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for SPAG9.

• Reactivity: Hu
• Applications: WB

SPAG9 Overexpression Lysate Summary

• Description:

  SPAG9 Transient Overexpression Lysate

  
  Expression Host: HEK293T
  
  Plasmid: RC217252
  
  Accession#: NM_003971
  
  Protein Tag: C-MYC/DDK
  
  You will receive 1 vial of lysate (100ug), 1 vial of empty vector negative control (100ug), and 1 vial of 2xSDS sample buffer (250ul). Each vial of cell lysate contains 100ug of total protein (at 1 mg/ml). The 2xSDS Sample Buffer consists of 4% SDS, 125mM Tris-HCl pH6.8, 10% Glycerol, 0.002% Bromophenol blue, 100mM DTT.
• Gene: SPAG9

Applications/Dilutions

• Dilutions:
  • Western Blot
• Application Notes: This product is intended for use as a positive control in Western Blot. Overexpression of the target protein was confirmed using an antibody to DDK (FLAG) epitope tag (NBP1-71705) present on the protein construct.
  
  Each vial of cell lysate contains 100ug of total protein which should be sufficient for 20-50 reactions. Depending on over-expression level, antibody affinity and detection system, some lysates can go as low as 0.1 ug per load. We recommend starting with 5ug of cell lysate. Add an equal amount of cell lysate and 2X SDS Sample buffer and boil the SDS samples for 10 minutes before loading.
• Theoretical MW: 144.5 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -80C. Avoid freeze-thaw cycles.
• Buffer: RIPA buffer

Lysate Details for Array

• Type: Overexpression

Notes

HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.

Alternate Names for SPAG9 Overexpression Lysate

• Cancer/testis antigen 89
• c-Jun NH2-terminal kinase-associated leucine zipper protein
• C-Jun-amino-terminal kinase-interacting protein 4
• CT89JIP4
• FLJ14006
• FLJ26141
• FLJ34602
• HLC4
• HLC-6
• HSS
• Human lung cancer oncogene 6 protein
• JLPPHETSYD1FLJ13450
• JNK interacting protein
• JNK/SAPK-associated protein
• JNK-associated leucine-zipper protein
• JNK-interacting protein 4
• KIAA0516JIP-4
• lung cancer oncogene 4
• MAPK8IP4
• Max-binding protein
• MGC117291
• MGC14967
• MGC74461
• Mitogen-activated protein kinase 8-interacting protein 4
• PIG6
• proliferation-inducing gene 6
• Proliferation-inducing protein 6
• Protein highly expressed in testis
• sperm associated antigen 9
• Sperm surface protein
• Sperm-associated antigen 9
• Sperm-specific protein
• Sunday driver 1

Background

Extracellular signals are transduced into cells through mitogen-activated protein kinases. The structural organization of these kinases into specific signaling domains is facilitated by scaffolding proteins involved in closely tethering different kinases so that successive phosphorylation events can occur. The protein encoded by this gene is a scaffolding protein that brings together mitogen-activated protein kinases and their transcription factor targets for the activation of specific signaling pathways. This gene which is abundantly expressed in testicular haploid germ cells encodes a protein that is recognized by sperm-agglutinating antibodies and implicated in infertility.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

Bioinformatics

• Gene Symbol: SPAG9