SPAG16 Overexpression Lysate (Native)

Images

 
Western Blot: SPAG16 Overexpression Lysate (Adult Normal) [NBP2-06322] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for SPAG16.

Product Details

Summary
Applications WB

Order Details

SPAG16 Overexpression Lysate (Native) Summary

Immunogen
The lysate was created in HEK293T cells, using plasmid ID RC215316 and based on accession number NP_078808. The protein contains a C-terminal DDK tag.
Specificity
Homo sapiens SPAG16, mRNA.
Gene
SPAG16

Packaging, Storage & Formulations

Storage
Store at -80C. Avoid freeze-thaw cycles.
Conjugate
Unconjugated
Buffer
RIPA buffer

Applications/Dilutions

Application Notes
This product is intended for use as a positive control in Western Blot. You will receive the lysate (100ug), and an empty vector negative control (100 ug).
Theoretical MW
70.6 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Lysate Details for SPAG16

Type
Overexpression
Protein State
Native

Notes

HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.This product is manufactured by and sold under license from OriGene Technologies and its use is limited solely for research purposes.

Alternate Names for SPAG16 Overexpression Lysate (Native)

  • DKFZp666P1710
  • FLJ22724
  • FLJ37717
  • Pf20 protein homolog
  • PF20MGC87036
  • sperm associated antigen 16
  • sperm-associated antigen 16 protein
  • sperm-associated WD repeat protein
  • WD repeat domain 29
  • WDR29

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

Customers Who Viewed This Item Also Viewed...

NBP1-80923
Species: Hu
Applications: WB, IHC, IHC-P
NB100-360
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC, IHC-P, IP
NB100-1774
Species: Hu, Rt, Am, Dr
Applications: WB, EM, ELISA, ICC/IF, IP
NB120-11012
Species: Hu, Bv(-), Ca(-), Eq(-), Gp(-), Mu(-), Po(-)
Applications: WB, ELISA, ICC/IF, IP
NBP1-93841
Species: Hu
Applications: ICC/IF, IHC, IHC-P
NBP2-36776
Species: Hu
Applications: WB, ICC/IF, IHC
NB100-410
Species: Hu, Mu
Applications: WB, IP
NBP1-84466
Species: Hu
Applications: WB, ICC/IF, IHC, IHC-P
NBP1-94132
Species: Hu
Applications: ICC/IF, IHC, IHC-P
H00057106-Q01
Species: Hu
AF1936
Species: Hu
Applications: WB, IP
NBP1-81637
Species: Hu
Applications: WB, IHC, IHC-P
FAB11311P
Species: Hu
Applications: Flow
NBP2-24582
Species: Hu, Mu, Rt, Pm
Applications: WB, IHC-P
NB300-213
Species: Hu, Mu, Rt, Bv
Applications: WB, ICC/IF, IHC, IHC-Fr
NBP2-47266
Species: Hu
Applications: ICC/IF, IHC, IHC-P, IF
AF1924
Species: Hu
Applications: WB, Simple Western, ChIP, ICC
NBP1-88527
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC, IHC-P

Publications for SPAG16 Lysate (NBP2-06322) (0)

There are no publications for SPAG16 Lysate (NBP2-06322).
By submitting your publication information earn gift cards and discounts for future purchases.

Reviews for SPAG16 Lysate (NBP2-06322) (0)

There are no reviews for SPAG16 Lysate (NBP2-06322). By submitting a review earn points towards our Rewards Program.
  • 250 points for product review
  • 500 additional points for an image with your product review
  • Double points (500) if you are the first to review this product
  • Double points (1000) if you are the first to review this product with an image

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for SPAG16 Lysate (NBP2-06322) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Additional SPAG16 Overexpression Lysate (Native) Products

Related Products by Gene

Bioinformatics Tool for SPAG16 Lysate (NBP2-06322)

Discover related pathways, diseases and genes to SPAG16 Lysate (NBP2-06322). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for SPAG16 Lysate (NBP2-06322)

Discover more about diseases related to SPAG16 Lysate (NBP2-06322).
 

Pathways for SPAG16 Lysate (NBP2-06322)

View related products by pathway.

Blogs on SPAG16

There are no specific blogs for SPAG16, but you can read our latest blog posts.

Contact Information

Product PDFs

Bioinformatics

Gene Symbol SPAG16

Customer Resources

Novus Review - Submit your review and earn rewards points which can be used for merchandise & discounts.
Risk Free Testing - Test on a species/application not listed above to receive a full credit towards a future purchase.

Novus' Quality Guarantee - Novus guarantees that every product we sell will work in the application and species listed on our website and datasheets.

Submit your question on NBP2-06322 below.
During business hours, we will respond to your email within 24 hours. For any questions submitted on the weekend, a response will be received on Monday.
For immediate assistance during business hours M- F (excluding major holidays), please contact us.
Ask a Scientist - We have a lab full of white coats just waiting for your scientific questions and concerns.

Customers Who Bought This Also Bought

SPAG16 Recombinant Protein
H00079582-P01-10ug
SPAG16 Antibody
NBP1-84124
SPAG16 Antibody
NBP1-84124
SPAG16 Antibody
NBP1-84124
MAP2 Antibody
NB300-213
MAP2 Antibody
NB300-213
MAP2 Antibody
NB300-213