![Western Blot: SPACA4 Antibody [NBP2-38913] - Analysis in control (vector only transfected HEK293T lysate) and SPACA4 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).](http://images.novusbio.com/fullsize/SPACA4-Antibody-Western-Blot-NBP2-38913-img0005.jpg "Western Blot: SPACA4 Antibody [NBP2-38913] - Analysis in control (vector only transfected HEK293T lysate) and SPACA4 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).")
| Reactivity | HuSpecies Glossary |
| Applications | WB, IHC |
| Clonality | Polyclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Format | BSA Free |
| Description | Novus Biologicals Rabbit SPACA4 Antibody - BSA Free (NBP2-38913) is a polyclonal antibody validated for use in IHC and WB. All Novus Biologicals antibodies are covered by our 100% guarantee. |
| Immunogen | This antibody was developed against a recombinant protein corresponding to amino acids: GVKDCVFCELTDSMQCPGTYM |
| Isotype | IgG |
| Clonality | Polyclonal |
| Host | Rabbit |
| Gene | SPACA4 |
| Purity | Affinity purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Application Notes | For IHC-Paraffin, HIER pH 6 retrieval is recommended. |
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| Control Peptide |
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| Storage | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
| Buffer | PBS (pH 7.2) and 40% Glycerol |
| Preservative | 0.02% Sodium Azide |
| Purity | Affinity purified |
Secondary Antibodies |
Isotype Controls |
The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.
![Immunohistochemistry-Paraffin: SPACA4 Antibody [NBP2-38913] - Staining of human colon.](http://images.novusbio.com/fullsize/SPACA4-Antibody-Immunohistochemistry-Paraffin-NBP2-38913-img0009.jpg "Immunohistochemistry-Paraffin: SPACA4 Antibody [NBP2-38913] - Staining of human colon.")
![Immunohistochemistry-Paraffin: SPACA4 Antibody [NBP2-38913] - Staining of human endometrium shows low expression as expected.](http://images.novusbio.com/fullsize/SPACA4-Antibody-Immunohistochemistry-Paraffin-NBP2-38913-img0002.jpg "Immunohistochemistry-Paraffin: SPACA4 Antibody [NBP2-38913] - Staining of human endometrium shows low expression as expected.")
![Immunohistochemistry-Paraffin: SPACA4 Antibody [NBP2-38913] - Staining of human testis shows high expression.](http://images.novusbio.com/fullsize/SPACA4-Antibody-Immunohistochemistry-Paraffin-NBP2-38913-img0003.jpg "Immunohistochemistry-Paraffin: SPACA4 Antibody [NBP2-38913] - Staining of human testis shows high expression.")
![Immunohistochemistry-Paraffin: SPACA4 Antibody [NBP2-38913] - Staining in human testis and endometrium tissues using anti-SPACA4 antibody. Corresponding SPACA4 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/SPACA4-Antibody-Immunohistochemistry-Paraffin-NBP2-38913-img0004.jpg "Immunohistochemistry-Paraffin: SPACA4 Antibody [NBP2-38913] - Staining in human testis and endometrium tissues using anti-SPACA4 antibody. Corresponding SPACA4 RNA-seq data are presented for the same tissues.")
![Immunohistochemistry-Paraffin: SPACA4 Antibody [NBP2-38913] - Staining of human cerebral cortex, colon, lymph node and testis using Anti-SPACA4 antibody NBP2-38913 (A) shows similar protein distribution across tissues to independent antibody NBP1-80793 (B).](http://images.novusbio.com/fullsize/SPACA4-Antibody-Immunohistochemistry-Paraffin-NBP2-38913-img0006.jpg "Immunohistochemistry-Paraffin: SPACA4 Antibody [NBP2-38913] - Staining of human cerebral cortex, colon, lymph node and testis using Anti-SPACA4 antibody NBP2-38913 (A) shows similar protein distribution across tissues to independent antibody NBP1-80793 (B).")
![Immunohistochemistry-Paraffin: SPACA4 Antibody [NBP2-38913] - Staining of human lymph node.](http://images.novusbio.com/fullsize/SPACA4-Antibody-Immunohistochemistry-Paraffin-NBP2-38913-img0007.jpg "Immunohistochemistry-Paraffin: SPACA4 Antibody [NBP2-38913] - Staining of human lymph node.")
![Immunohistochemistry-Paraffin: SPACA4 Antibody [NBP2-38913] - Staining of human cerebral cortex.](http://images.novusbio.com/fullsize/SPACA4-Antibody-Immunohistochemistry-Paraffin-NBP2-38913-img0008.jpg "Immunohistochemistry-Paraffin: SPACA4 Antibody [NBP2-38913] - Staining of human cerebral cortex.")
![N/A uPAR [HRP]](https://images.novusbio.com/images2/DATA_uPAR_DUP00_ELISA_866.jpg)
![N/A uPAR [HRP]](https://images.novusbio.com/images2/uPAR_DUP00_ELISA_203.jpg)
![Enzyme Activity u-Plasminogen Activator/Urokinase [Unconjugated]](https://images.novusbio.com/images2/uPlasminogen_Activator_1310SE_1837.jpg)
![Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).](https://images.novusbio.com/images/Goat-anti-Rabbit-IgG-H+L-Secondary-Antibody-HRP-Western-Blot-NB7160-img0001.jpg "Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).")
![Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.](https://images.novusbio.com/images/Rabbit--Mouse-IgG-Isotype-Control-Flow-Cytometry-NBP2-24891-img0001.jpg "Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.")