SP100 Antibody (PCRP-SP100-1B9) - Azide and BSA Free

Protein Array: SP100 Antibody (PCRP-SP100-1B9) - Azide and BSA Free [NBP3-14183] - Analysis of Protein Array containing more than 19,000 full-length human proteins using SP100 Antibody (PCRP-SP100-1B9).

Immunofluorescence Analysis of PFA-fixed HeLa cells stained using SP100 Antibody (PCRP-SP100-1B9) - Azide and BSA Free followed by goat anti-mouse IgG-CF488. Membrane stained with phalloidin.

IHC analysis of formalin-fixed, paraffin-embedded human colon. Strong nuclear staining using SP100 Antibody (PCRP-SP100-1B9) - Azide and BSA Free at 2ug/ml in PBS for 30min RT. HIER: Tris/EDTA, pH9.0, 45min. 2 °: HRP-polymer, 30min. DAB, 5min.

Flow cytometric analysis of PFA-fixed HeLa cells. SP100 Antibody (PCRP-SP100-1B9) - Azide and BSA Free followed by goat anti-mouse IgG-CF488 (blue); unstained cells (red).

IHC analysis of formalin-fixed, paraffin-embedded human tonsil. Strong nuclear staining using SP100 Antibody (PCRP-SP100-1B9) - Azide and BSA Free at 2ug/ml in PBS for 30min RT. HIER: Tris/EDTA, pH9.0, 45min. 2 °: HRP-polymer, 30min. DAB, 5min.

• Reactivity: Hu
• Applications: WB, ELISA, Flow, ICC/IF, IHC, IP, MA
• Clone: PCRP-SP100-1B9
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free
• Concentration: 1 mg/ml

SP100 Antibody (PCRP-SP100-1B9) - Azide and BSA Free Summary

• Description: 1.0 mg/ml of antibody purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS WITHOUT BSA & azide. Also available at 200 ug/ml WITH BSA & azide (NBP3-13780).
  
  Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80 C.
• Immunogen: Recombinant full-length human SP100 protein (Uniprot: P23497)
• Localization: Nucleus, PML body
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Gene: SP100
• Purity: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Flow Cytometry 0.5-2 ug/million cells
  • Immunocytochemistry/ Immunofluorescence 0.5-2 ug/ml
  • Immunohistochemistry-Paraffin 1-2 ug/ml
  • Immunoprecipitation 1-2 ug per 100-500 ug protein
  • Protein Array
  • Western Blot 1-2 ug/ml
• Application Notes: ELISA: For coating, order this antibody without BSA
  
  Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes
  
  Immunoprecipitation: 1-2ug per 100-500ug of total protein (1ml of cell lysate)
• Theoretical MW: 100.4 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -20 to -80C. Avoid freeze-thaw cycles.
• Buffer: 10mM PBS
• Preservative: No Preservative
• Concentration: 1 mg/ml
• Purity: Protein A or G purified

Alternate Names for SP100 Antibody (PCRP-SP100-1B9) - Azide and BSA Free

• DKFZp686E07254
• FLJ00340
• FLJ34579
• lysp100b
• nuclear antigen Sp100
• nuclear autoantigen Sp-100
• Nuclear dot-associated Sp100 protein
• SP100 nuclear antigen
• SP100-HMG nuclear autoantigen
• Speckled 100 kDa

Background

SP100 may play a role in the control of gene expression

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: SP100