SNAP29 Antibody (3E4-E6) - Azide and BSA Free

Western Blot: SNAP29 Antibody (3E4-E6) [H00009342-M01] - Analysis of SNAP29 expression in transfected 293T cell line by SNAP29 monoclonal antibody (M01), clone 3E4-E6.Lane 1: SNAP29 transfected lysate(28.38 KDa).Lane 2: Non-transfected lysate.

Immunocytochemistry/Immunofluorescence: SNAP29 Antibody (3E4-E6) [H00009342-M01] - Analysis of monoclonal antibody to SNAP29 on HeLa cell . Antibody concentration 10 ug/ml.

Sandwich ELISA: SNAP29 Antibody (3E4-E6) [H00009342-M01] - Detection limit for recombinant GST tagged SNAP29 is 0.1 ng/ml as a capture antibody.

• Reactivity: Hu
• Applications: WB, ELISA, ICC/IF
• Clone: 3E4-E6
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free

SNAP29 Antibody (3E4-E6) - Azide and BSA Free Summary

• Immunogen: SNAP29 (AAH09715, 1 a.a. ~ 258 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MSAYPKSYNPFDDDGEDEGARPAPWRDARDLPDGPDAPADRQQYLRQEVLRRAEATAASTSRSLALMYESEKVGVASSEELARQRGVLERTEKMVDKMDQDLKISQKHINSIKSVFGGLVNYFKSKPVETPPEQNGTLTSQPNNRLKEAISTSKEQEAKYQASHPNLRKLDDTDPVPRGAGSAMSTDAYPKNPHLRAYHQKIDSNLDELSMGLGRLKDIALGMQTEIEEQDDILDRLTTKVDKLDVNIKSTERKVRQL
• Specificity: SNAP29 - synaptosomal-associated protein, 29kDa
• Isotype: IgG1 Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: SNAP29
• Purity: IgG purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Immunocytochemistry/ Immunofluorescence
  • Sandwich ELISA
  • Western Blot 1:500
• Application Notes: Antibody reactive against transfected lysate and recombinant protein for western blot. It has also been used for ELISA.

Reactivity Notes

Human. Other species not tested.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: In 1x PBS, pH 7.4
• Preservative: No Preservative
• Purity: IgG purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for SNAP29 Antibody (3E4-E6) - Azide and BSA Free

• CEDNIK
• CEDNIKSoluble 29 kDa NSF attachment protein
• SNAP29
• SNAP-29FLJ21051
• synaptosomal-associated protein 29
• synaptosomal-associated protein, 29kD
• synaptosomal-associated protein, 29kDa
• Vesicle-membrane fusion protein SNAP-29

Background

This gene, a member of the SNAP25 gene family, encodes a protein involved in multiple membrane trafficking steps. Two other members of this gene family, SNAP23 and SNAP25, encode proteins that bind a syntaxin protein and mediate synaptic vesicle membrane docking and fusion to the plasma membrane. The protein encoded by this gene binds tightly to multiple syntaxins and is localized to intracellular membrane structures rather than to the plasma membrane. While the protein is mostly membrane-bound, a significant fraction of it is found free in the cytoplasm. Use of multiple polyadenylation sites has been noted for this gene.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for SNAP29 Antibody (H00009342-M01)(2)

Publications using H00009342-M01

• Pattu V, Qu B, Schwarz EC et al. SNARE protein expression and localization in human cytotoxic T lymphocytes. Eur J Immunol. 2011-11-25 [PMID: 22120889]
• Larkin C Examining the TRPV3 cation channel in a culture model of human epidermis Thesis 2023-01-01 (WB, ICC/IF, Human)

Bioinformatics

• Gene Symbol: SNAP29
• Entrez:
  • 9342(Human)
• OMIM:
  • 604202(Human)
• Uniprot:
  • AAH09715(Human)