SNAP47 Antibody - BSA Free

Immunohistochemistry-Paraffin: SNAP47 Antibody [NBP1-88483] - Staining in human endometrium and pancreas tissues using anti-SNAP47 antibody. Corresponding SNAP47 RNA-seq data are presented for the same tissues.

Immunocytochemistry/Immunofluorescence: SNAP47 Antibody [NBP1-88483] - Staining of human cell line U-2 OS shows positivity in cytoplasm.

Immunohistochemistry-Paraffin: SNAP47 Antibody [NBP1-88483] - Staining of human endometrium shows high expression.

Immunohistochemistry-Paraffin: SNAP47 Antibody [NBP1-88483] - Staining of human pancreas shows low expression as expected.

• Reactivity: Hu
• Applications: ICC/IF, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

SNAP47 Antibody - BSA Free Summary

• Description: Novus Biologicals Rabbit SNAP47 Antibody - BSA Free (NBP1-88483) is a polyclonal antibody validated for use in IHC and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: This antibody was developed against Recombinant Protein corresponding to amino acids: DDIKVHSPYEISIRQRFIGKPDMAYRLISAKMPEVIPILEVQFSKKMELLEDALVLRSARTSSPAEKSCSVWHAASGLMGRTLHREPPAGDQE
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: SNAP47
• Purity: Affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 0.25-2 ug/ml
  • Immunohistochemistry 1:20 - 1:50
  • Immunohistochemistry-Paraffin 1:20 - 1:50
• Application Notes: For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF,Fixation Permeabilization: Use PFA/Triton X-100.
• Control Peptide:

  SNAP47 Protein (NBP1-88483PEP)

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Rat (81%)

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.2) and 40% Glycerol
• Preservative: 0.02% Sodium Azide
• Purity: Affinity purified

Alternate Names for SNAP47 Antibody - BSA Free

• C1orf142
• Epididymis luminal protein 170
• ESFI5812
• FLJ12517
• HEL170
• HEL-S-290
• SNAP47
• SNAP-47chromosome 1 open reading frame 142
• SVAP1
• SVAP1DKFZp686M10160
• Synaptosomal-associated 47 kDa protein
• synaptosomal-associated protein 47
• synaptosomal-associated protein, 47kDa

Background

SNAP47 belongs to the SVAP1 family. It contains two t-SNARE coiled-coil homology domains. SNAP47 has ubiquitous tissue distribution, with particularly high levels in nervous tissue. In neurons, SNAP 47 has been shown to have widespread distribution on intracellular membranes and is enriched in synaptic vesicle fractions. In vitro, SNAP 47 can substitute for SNAP-25 in SNARE complex formation, but not as efficiently.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: SNAP47