Slo3 Antibody - Azide and BSA Free

Western blot shows 40 μg of RIPA lysates from HEK 293 control (-) and overexpressing Slo3 (+). Nitrocellulose membrane was probed with Slo3 Antibody - Azide and BSA Free (Catalog # NBP3-05611) followed by HRP-conjugated goat anti-Rabbit IgG Secondary Antibody. A specific band was detected for Slo3 at approximately 138 kDa (as indicated) in the overexpressing Slo3 cell line. Primary antibody dilution used: 1/1000. The Ponceau stained transfer of the blot is shown. This experiment was conducted under reducing conditions. Image, protocol, and testing courtesy of YCharOS Inc. See ycharos.com for additional details.

Western Blot: Slo3 Antibody [NBP3-05611] - Western blot analysis of extracts of 25 ug rat testis, using (NBP3-05611) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Blocking buffer: 3% nonfat dry milk in TBST. Exposure time: 90s.

• Reactivity: Rt
• Applications: WB, ELISA
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: Azide and BSA Free

Slo3 Antibody - Azide and BSA Free Summary

• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 920-1149 of human Slo3 (NP_001027006.2). LELLQMLVTGGVSSQLEQHLDKDKVYGVADSCTSLLSGRNRCKLGLLSLHETILSDVNPRNTFGQLFCGSLDLFGILCVGLYRIIDEEELNPENKRFVITRPANEFKLLPSDLVFCAIPFSTACYKRNEEFSLQKSYEIVNKASQTTETHSDTNCPPTIDSVTETLYSPVYSYQPRTNSLSFPKQIAWNQSRTNSIISSQIPLGDNAKENERKTSDEVYDEDPFAYSEPL
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: KCNU1
• Purity: Affinity purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Western Blot 1:500-1:2000
• Theoretical MW: 129 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.3), 50% glycerol
• Preservative: 0.01% Thimerosal
• Purity: Affinity purified

Alternate Names for Slo3 Antibody - Azide and BSA Free

• Calcium-activated potassium channel subunit alpha-3
• KCa5
• KCa5.1
• Kcnma3
• KCNMC1Calcium-activated potassium channel, subfamily M subunit alpha-3
• potassium channel subfamily U member 1
• potassium channel, subfamily U, member 1
• Slo3
• Slowpoke homolog 3

Background

Slo3 is a novel potassium channel expressed in mammalian spermatocytes. Slo3 regulates both intracellular pH and membrane voltage. Slo3 might play a role in alkalization-mediated K+ fluxes associated with sperm capacitation.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including Methotrexate, which is known to the State of California to cause reproductive toxicity with developmental effects. For more information, go to www.P65Warnings.ca.gov

Bioinformatics

• Gene Symbol: KCNU1