SLC14A1 Antibody (888418)

Human red blood cells were stained with Mouse Anti-Human SLC14A1 Monoclonal Antibody (Catalog # MAB8238, filled histogram) or isotype control antibody (Catalog # MAB0041, open histogram), followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B).

HEK293 human embryonic kidney cell line transfected with human SLC14A1 and eGFP was stained with either (A) Mouse Anti-Human SLC14A1 Monoclonal Antibody (Catalog # MAB8238) or (B) Mouse IgG2B Flow Cytometry Isotype Control (Catalog # MAB0041) followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B).

• Reactivity: Hu
• Applications: Flow, CyTOF-ready
• Clone: 888418
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: LYOPH

SLC14A1 Antibody (888418) Summary

• Immunogen: NS0 mouse myeloma cell line transfected with human SLC14A1
  Accession # Q13336
• Specificity: Detects human SLC14A1 in direct ELISAs.
• Source: N/A
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Gene: SLC14A1
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • CyTOF-ready
  • Flow Cytometry 2.5 ug/10^6 cells

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for SLC14A1 Antibody (888418)

• FLJ33745
• HsT1341
• HUT11
• JK
• JKFLJ41687
• RACH1
• RACH2
• SLC14A1
• solute carrier family 14 (urea transporter), member 1 (Kidd blood group)
• Solute carrier family 14 member 1
• truncated urea transporter
• urea transporter 1
• urea transporter JK glycoprotein
• Urea transporter, erythrocyte
• urea transporter-B1
• UT1
• UT1UT-B1
• UT-B1
• UTE
• UTEblood group Kidd urea transporter

Background

Urea transporter 1, designated Solute Carrier family 14 member 1 (SLC14A1) is a 43-54 kDa multipass urea transporter mainly expressed in erythrocytes, but also found in kidney, mesenchymal stem cells, and some carcinomas. It contains an epitope identified as the Kidd (JK) blood group antigen. The antigenic forms differ at only one amino acid (aa); Asp280 represents Jk(a) and Asn280 represents Jk(b). SLC14A1 Isoform 2 (445 aa) contains an extended N-terminus as compared to Isoform 1 (389 aa). Human SLC14A1 shares 83% and 81% aa sequence identity with mouse and rat SLC14A1, respectively.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: SLC14A1
• Uniprot:
  • Q13336()