Slap Antibody

Immunohistochemistry-Paraffin: Slap Antibody [NBP2-24696] - Formalin-fixed, paraffin-embedded human kidney stained with SLAP antibody at 15 ug/ml. Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 10 mM sodium citrate buffer, pH 6.0 for 10-20 min followed by cooling at RT for 20 min.

Product Discontinued

Slap Antibody Summary

• Immunogen: A portion of amino acids 175-225 of human SLAP was used as the immunogen for the antibody.
• Predicted Species: Chimpanzee (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: SLA
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 5-15 ug/ml

Reactivity Notes

The SLAP amino acid sequence used as immunogen for the antibody is 100% homologous in human (isoforms a, b and c) and chimpanzee, 83% homologous in mouse and 78% homologous in rat and bovine.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: 0.2 ml PBS and 0.05% BSA
• Preservative: 0.05% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Protein A purified

Alternate Names for Slap Antibody

• hSLAP
• SLA1
• SLAP
• SLAP1
• SLAP-1
• Src-like-adapter protein 1
• Src-like-adapter
• Src-like-adaptor

Background

SLAP also known as Src-like-adapter protein 1 is a 276 amino acid containing adapter protein, functioning as a negative regulator of T-cell receptor (TCR) signaling. SLAP belonging to the SRC family of cytoplasmic tyrosine kinases contains a SH2 domain and a SH3 domain. This cytoplasmic protein colocalizes with endosomes and is known to inhibit T-cell antigen-receptor induced activation of nuclear factor of activated T-cells. It acts as a negative regulator of positive selection and mitosis of T-cells. SLAP may act by linking signaling proteins such as ZAP70 with CBL, leading to a CBL dependent degradation of signaling proteins. Expression of SLAP is weak in heart, adult brain, placenta, liver, skeletal muscle, kidney and pancreas but is high in lung and fetal brain.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Slap Antibody (NBP2-24696)(1)

Publication using NBP2-24696

• Naudin C, Sirvent A, Leroy C et al. SLAP displays tumour suppressor functions in colorectal cancer via destabilization of the SRC substrate EPHA2. Nat Commun. 2014-01-24 [PMID: 24457997] (IHC-P, Human)

Bioinformatics

• Gene Symbol: SLA