SKA1 Overexpression Lysate

Images

 
Western Blot: SKA1 Overexpression Lysate (Adult Normal) [NBL1-08252] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for SKA1.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB

Order Details

SKA1 Overexpression Lysate Summary

Description

SKA1 Transient Overexpression Lysate


Expression Host: HEK293T

Plasmid: RC202370

Accession#: NM_145060

Protein Tag: C-MYC/DDK
Gene
SKA1

Applications/Dilutions

Dilutions
  • Western Blot
Application Notes
This product is intended for use as a positive control in Western Blot.

You will receive 1 vial of lysate (100ug), 1 vial of empty vector negative control (100ug), and 1 vial of 2xSDS sample buffer (250ul).
Theoretical MW
29.3 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -80C. Avoid freeze-thaw cycles.
Buffer
RIPA buffer

Lysate Details for Array

Type
Overexpression

Notes

HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.This product is manufactured by and sold under license from OriGene Technologies and its use is limited solely for research purposes.

Alternate Names for SKA1 Overexpression Lysate

  • C18orf24
  • chromosome 18 open reading frame 24
  • MGC10200
  • spindle and kinetochore associated complex subunit 1
  • spindle and kinetochore-associated protein 1
  • spindle and KT (kinetochore) associated 1

Background

SKA1 is a component of the SKA1 complex, a microtubule-binding subcomplex of the outer kinetochore that is essentialfor proper chromosome segregation. Required for timely anaphase onset during mitosis, when chromosomes undergo bipolarattachment on spindle microtubu

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol

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Bioinformatics

Gene Symbol SKA1