SKA1 Antibody

Western Blot: SKA1 Antibody [NBP1-72131] - SKA1 in A549 cell lysate with SKA1 antibody at (A) 0.5 and (B) 1 ug/ml.

Immunohistochemistry-Paraffin: SKA1 Antibody [NBP1-72131] - Analysis of anti-SKA1 antibody with human brain, cerebellum at concentration 5 ug/ml.

Immunohistochemistry-Paraffin: SKA1 Antibody [NBP1-72131] - Analysis of anti-SKA1 antibody with human brain, cortex at concentration 5 ug/ml.

Product Discontinued

SKA1 Antibody Summary

• Immunogen: A 13 amino acid synthetic peptide near the C-terminus of human SKA1. The immunogen is located within amino acids 180 - 230 of SKA1.
• Epitope: C-Terminus
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: SKA1
• Purity: Peptide affinity purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:100 - 1:2000
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 5 ug/ml
  • Western Blot 0.5-1 ug/ml

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.02% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Peptide affinity purified

Alternate Names for SKA1 Antibody

• C18orf24
• chromosome 18 open reading frame 24
• MGC10200
• spindle and kinetochore associated complex subunit 1
• spindle and kinetochore-associated protein 1
• spindle and KT (kinetochore) associated 1

Background

SKA1 is a component of the SKA1 complex, a microtubule-binding subcomplex of the outer kinetochore that is essentialfor proper chromosome segregation. Required for timely anaphase onset during mitosis, when chromosomes undergo bipolarattachment on spindle microtubu

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for SKA1 Antibody (NBP1-72131)(1)

Publication using NBP1-72131

• Radhakrishnan RM, Kizhakkeduth ST, Nair VM et al. Kinetochore-microtubule attachment in human cells is regulated by the interaction of a conserved motif of Ska1 with EB1 The Journal of biological chemistry 2022-12-30 [PMID: 36592928]

Bioinformatics

• Gene Symbol: SKA1