SFRS14 Antibody

Western Blot: SFRS14 Antibody [NB100-79849] - Detection of Human SFRS14 on HeLa whole cell lysate using NB100-79849. SFRS14 was also immunoprecipitated by rabbit anti-SFRS14 antibody NB100-79850.

Immunocytochemistry/Immunofluorescence: SFRS14 Antibody [NB100-79849] - FFPE section of human prostate carcinoma. Affinity purified rabbit anti-SFRS14 used at a dilution of 1:100. Detection: Red-fluorescent goat anti-rabbit IgG highly cross-adsorbed Antibody Hilyte PlusTM 555 used at a dilution of 1:100.

Immunohistochemistry: SFRS14 Antibody [NB100-79849] - Sample: FFPE section of human Ewing sarcoma. Antibody: Affinity purified rabbit anti- SFRS14 used at a dilution of 1:200 (1ug/ml). Detection: DAB

Immunohistochemistry-Paraffin: SFRS14 Antibody [NB100-79849] - FFPE section of human prostate carcinoma. Affinity purified rabbit anti-SFRS14 used at a dilution of 1:250. Detection: DAB staining using Immunohistochemistry Accessory Kit

Immunohistochemistry-Paraffin: SFRS14 Antibody [NB100-79849] - FFPE section of human pancreatic islet cell tumor. Affinity purified rabbit anti-SFRS14 used at a dilution of 1:250. Detection: DAB staining using Immunohistochemistry Accessory Kit

• Reactivity: Hu
• Applications: WB, ICC/IF, IHC, IP
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Concentration: 0.2 mg/ml

SFRS14 Antibody Summary

• Immunogen: The immunogen recognized by this antibody maps to a region between residue 725 and 775 of human splicing factor, arginine/serine-rich 14 using the numbering given in entry NP_055699.1 (GeneID 10147).
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: SUGP2
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:250- 1:1000
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:100-1:500
  • Immunoprecipitation 2-5 mcg/mg lysate
  • Western Blot 1:2000-1:10000

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: TBS and 0.1% BSA
• Preservative: 0.09% Sodium Azide
• Concentration: 0.2 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for SFRS14 Antibody

• Arginine/serine-rich-splicing factor 14
• DKFZp686M2322
• DKFZp779L2418
• KIAA0365arginine/serine-rich 14 splicing factor
• putative splicing factor, arginine/serine-rich 14
• SFRS14
• Splicing factor, arginine/serine-rich 14FLJ21855
• SRFS14
• SURP and G patch domain containing 2
• SURP and G-patch domain-containing protein 2

Background

SFRS14 (splicing factor arginine/serine-rich 14) is a putative alternative splicing factor. It contains two SURP motif repeats which are found among proteins involved in regulated mRNA splicing and a G-patch domain which features six highly conserved glycine residues found in several RNA-associated proteins. SFRS14 also bears an N-terminal arginine/serine-rich region found among the SR-related family of pre-mRNA processing factors.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: SUGP2
• Entrez:
  • 10147(Human)
• Uniprot:
  • NP_055699.1(Human)