SFMBT1 Overexpression Lysate

Images

 
Western Blot: SFMBT1 Overexpression Lysate (Adult Normal) [NBP2-08909] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for SFMBT1.

Product Details

Summary
Product Discontinued
View other related SFMBT1 Lysates

Order Details


    • Catalog Number
      NBP2-08909
    • Availability
      Product Discontinued

    Can't find what you are looking for? Use our Antibody Concierge Service & we will help you locate your antibody!

    Or feel free to contact us for alternative products.

SFMBT1 Overexpression Lysate Summary

Description
Transient overexpression lysate of Scm-like with four mbt domains 1 (SFMBT1), transcript variant 1 The lysate was created in HEK293T cells, using Plasmid ID RC201019 and based on accession number NM_001005159. The protein contains a C-MYC/DDK Tag. You will receive 1 vial of lysate (100ug), 1 vial of empty vector negative control (100ug), and 1 vial of 2xSDS sample buffer (250ul). Each vial of cell lysate contains 100ug of total protein (at 1 mg/ml). The 2xSDS Sample Buffer consists of 4% SDS, 125mM Tris-HCl pH6.8, 10% Glycerol, 0.002% Bromophenol blue, 100mM DTT.
Gene
SFMBT1

Applications/Dilutions

Dilutions
  • Western Blot
Application Notes
This product is intended for use as a positive control in Western Blot. Overexpression of the target protein was confirmed using an antibody to DDK (FLAG) epitope tag (NBP1-71705) present on the protein construct.

Each vial of cell lysate contains 100ug of total protein which should be sufficient for 20-50 reactions. Depending on over-expression level, antibody affinity and detection system, some lysates can go as low as 0.1 ug per load. We recommend starting with 5ug of cell lysate. Add an equal amount of cell lysate and 2X SDS Sample buffer and boil the SDS samples for 10 minutes before loading.
Theoretical MW
98 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -80C. Avoid freeze-thaw cycles.
Buffer
RIPA buffer

Lysate Details for Array

Type
Overexpression

Notes

HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.This product is manufactured by and sold under license from OriGene Technologies and its use is limited solely for research purposes.

Alternate Names for SFMBT1 Overexpression Lysate

  • DKFZp434L243
  • Renal ubiquitous protein 1
  • RU1scm-like with four MBT domains protein 1
  • Scm-like with four mbt domains 1
  • Scm-related gene containing four mbt domains
  • Scm-related gene product containing four mbt domains
  • SFMBT

Background

SFMBT1 shares high similarity with the Drosophila Scm (sex comb on midleg) gene. It encodes a protein which contains four malignant brain tumor repeat (mbt) domains and may be involved in antigen recognition. Several alternative splice variants have been characterized. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

Customers Who Viewed This Item Also Viewed...

291-G1
Species: Hu
Applications: BA
NBP2-32020
Species: Hu
Applications: IHC,  IHC-P
NBP1-25966
Species: Hu
Applications: Flow, ICC/IF, IHC, IHC-Fr,  IHC-P, WB
NBP1-84298
Species: Hu, Mu
Applications: ICC/IF, IHC,  IHC-P, WB
NB500-171
Species: Hu, I, Mu, Rt, Xp, Ye
Applications: ChIP, IB, ICC/IF, IHC,  IHC-P, Single-Cell Western, WB
NB600-488
Species: Hu, Mu
Applications: ICC/IF, IHC, IHC-Fr,  IHC-P, WB
NBP2-33470
Species: Hu
Applications: ICC/IF
NBP2-47579
Species: Hu
Applications: IB, ICC/IF, IHC,  IHC-P, WB
MAB1417
Species: Bv, Hu, Mu
Applications: CyTOF-ready, ICC, IHC, ICFlow
H00060485-M02
Species: Hu, Mu, Ye
Applications: ELISA, ICC/IF, IHC,  IHC-P, IP, WB
NBP2-07996
Species: Hu
Applications: WB
NBP2-97218
Species: Hu
Applications: ICC/IF,  IHC-P
NBP2-52486
Species: Hu
Applications: CyTOF-ready, ELISA, Flow, ICC/IF, IHC,  IHC-P, WB
NBP1-85260
Species: Hu
Applications: ChIP-EXO-SEQ, IHC,  IHC-P, WB
NBP1-85351
Species: Hu, Mu, Rt
Applications: ICC/IF, IHC,  IHC-P, WB
NBP2-08909
Species: Hu
Applications: WB

Publications for SFMBT1 Lysate (NBP2-08909) (0)

There are no publications for SFMBT1 Lysate (NBP2-08909).
By submitting your publication information earn gift cards and discounts for future purchases.

Reviews for SFMBT1 Lysate (NBP2-08909) (0)

There are no reviews for SFMBT1 Lysate (NBP2-08909). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for SFMBT1 Lysate (NBP2-08909) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Additional SFMBT1 Products

Blogs on SFMBT1

There are no specific blogs for SFMBT1, but you can read our latest blog posts.

Contact Information

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our SFMBT1 Overexpression Lysate and receive a gift card or discount.

Bioinformatics

Gene Symbol SFMBT1