SFMBT1 Overexpression Lysate

Images

 
Western Blot: SFMBT1 Overexpression Lysate (Adult Normal) [NBL1-15880] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for SFMBT1.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB

Order Details

SFMBT1 Overexpression Lysate Summary

Description
Transient overexpression lysate of Scm-like with four mbt domains 1 (SFMBT1), transcript variant 3 The lysate was created in HEK293T cells, using Plasmid ID RC218257 and based on accession number NM_016329. The protein contains a C-MYC/DDK Tag.
Gene
SFMBT1

Applications/Dilutions

Dilutions
  • Western Blot
Application Notes
This product is intended for use as a positive control in Western Blot.

You will receive 1 vial of lysate (100ug), 1 vial of empty vector negative control (100ug), and 1 vial of 2xSDS sample buffer (250ul).
Theoretical MW
98 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -80C. Avoid freeze-thaw cycles.
Buffer
RIPA buffer

Lysate Details for Array

Type
Overexpression

Notes

HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.This product is manufactured by and sold under license from OriGene Technologies and its use is limited solely for research purposes.

Alternate Names for SFMBT1 Overexpression Lysate

  • DKFZp434L243
  • Renal ubiquitous protein 1
  • RU1scm-like with four MBT domains protein 1
  • Scm-like with four mbt domains 1
  • Scm-related gene containing four mbt domains
  • Scm-related gene product containing four mbt domains
  • SFMBT

Background

This gene shares high similarity with the Drosophila Scm (sex comb on midleg) gene. It encodes a protein which contains four malignant brain tumor repeat (mbt) domains and may be involved in antigen recognition. Several alternative splice variants have been characterized. Transcript Variant: This variant (3) differs in the 5' UTR compared to variant 1; it contains an alternative first exon and excludes another exon in the 5' UTR. Variants 1, 2 and 3 encode the same protein.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

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Other Available Formats

NBL1-15880

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Bioinformatics

Gene Symbol SFMBT1