SETDB2 Antibody (1E2)

Western Blot: SETDB2 Antibody (1E2) [H00083852-M07] - Analysis of SETDB2 expression in MES-SA/Dx5 (Cat # L021V1).

Sandwich ELISA: SETDB2 Antibody (1E2) [H00083852-M07] - Detection limit for recombinant GST tagged SETDB2 is 3 ng/ml as a capture antibody.

Product Discontinued

SETDB2 Antibody (1E2) Summary

• Immunogen: SETDB2 (NP_114121.1, 620 a.a. ~ 719 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. KNTSSDSLTKFNKGNVFLLDATKEGNVGRFLNHSCCPNLLVQNVFVETHNRNFPLVAFFTNRYVKARTELTWDYGYEAGTVPEKEIFCQCGVNKCRKKIL
• Specificity: SETDB2 - SET domain, bifurcated 2 (1E2)
• Isotype: IgG2a Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: SETDB2
• Purity: Ascites

Applications/Dilutions

• Dilutions:
  • ELISA
  • Sandwich ELISA
  • Western Blot 1:500
• Application Notes: Antibody reactive against cell lysate for western blot. It has also been used for ELISA.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: In 1x PBS, pH 7.4
• Preservative: No Preservative
• Purity: Ascites

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for SETDB2 Antibody (1E2)

• C13orf4
• chromosome 13 open reading frame 4
• chronic lymphocytic leukemia deletion region 8
• Chronic lymphocytic leukemia deletion region gene 8 protein
• CLLD8DKFZp761J1217
• DKFZp586I0123
• EC 2.1.1.43
• histone-lysine N-methyltransferase SETDB2
• KMT1FCLLL8
• Lysine N-methyltransferase 1F
• SET domain bifurcated 2
• SET domain, bifurcated 2

Background

Proteins that contain a SET domain, such as SETDB2, modulate gene expression epigenetically through histone H3 (see MIM 601128) methylation. SETDB2 is likely a histone H3 methyltransferase, as it contains both the active site and flanking cysteine residues required for catalytic activity (Zhang et al., 2003 [PubMed 12754510]).[supplied by OMIM]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: SETDB2