SET Antibody (JB60-34)

Western Blot: SET Antibody (JB60-34) [NBP2-75661] - Analysis of SET on K562 cell lysates using anti-SET at 1/500 dilution.

Immunocytochemistry/Immunofluorescence: SET Antibody (JB60-34) [NBP2-75661] - Staining SET in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunohistochemistry-Paraffin: SET Antibody (JB60-34) [NBP2-75661] - Analysis of paraffin-embedded rat kidney tissue using anti-SET antibody. Counter stained with hematoxylin.

Immunocytochemistry/Immunofluorescence: SET Antibody (JB60-34) [NBP2-75661] - Staining SET in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunocytochemistry/Immunofluorescence: SET Antibody (JB60-34) [NBP2-75661] - Staining SET in LOVO cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunohistochemistry-Paraffin: SET Antibody (JB60-34) [NBP2-75661] - Analysis of paraffin-embedded human tonsil tissue using anti-SET antibody. Counter stained with hematoxylin.

Immunohistochemistry-Paraffin: SET Antibody (JB60-34) [NBP2-75661] - Analysis of paraffin-embedded human lung cancer tissue using anti-SET antibody. Counter stained with hematoxylin.

Immunohistochemistry-Paraffin: SET Antibody (JB60-34) [NBP2-75661] - Analysis of paraffin-embedded human kidney tissue using anti-SET antibody. Counter stained with hematoxylin.

Immunohistochemistry-Paraffin: SET Antibody (JB60-34) [NBP2-75661] - Analysis of paraffin-embedded mouse fallopian tube tissue using anti-SET antibody. Counter stained with hematoxylin.

Product Discontinued

SET Antibody (JB60-34) Summary

• Additional Information: Recombinant Monoclonal Antibody.
• Immunogen: Recombinant protein within Human SET aa 160-290 / 290. (SwissProt: Q01105 Human; SwissProt: Q9EQU5 Mouse)
• Localization: Nucleus. Cytoplasm. Endoplasmic reticulum.
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: SET
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 1:50-1:100
  • Immunocytochemistry/ Immunofluorescence 1:50-1:200
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1:50-1:200
  • Immunoprecipitation 1:10-1:50
  • Western Blot 1:500-1:2000
• Theoretical MW: 37 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: TBS (pH7.4), 0.05% BSA, 40% Glycerol
• Preservative: 0.05% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein A purified

Alternate Names for SET Antibody (JB60-34)

• 2PP2A
• HLA-DR-associated protein II
• I2PP2A
• I-2PP2A
• IGAAD
• Inhibitor of granzyme A-activated DNase
• inhibitor-2 of protein phosphatase-2A
• IPP2A2
• PHAPIITAF-I
• Phosphatase 2A inhibitor I2PP2A
• protein phosphatase type 2A inhibitor
• protein SET
• SET nuclear oncogene
• SET translocation (myeloid leukemia-associated)
• TAF-IBETA
• Template-activating factor I
• Template-Activating Factor-I, chromatin remodelling factor

Background

SET is a multi-functional protein involved in apoptosis, transcription, nucleosome assembly, and histone binding. SET was identified as part of a chromosomal rearrangement with NUP214/CAN in acute undifferentiated leukemia.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: SET