Serpin I1/Neuroserpin Antibody

Western Blot: Serpin I1/Neuroserpin Antibody [NBP1-31037] - Mouse tissue extract (50 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with Neuroserpin antibody [C3], C-term diluted at 1:500.

Immunocytochemistry/Immunofluorescence: Serpin I1/Neuroserpin Antibody [NBP1-31037] - Neuroserpin antibody [C3], C-term detects Neuroserpin protein by immunofluorescent analysis.Sample: DIV10 rat E18 primary cortical neuron cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: Neuroserpin stained by Neuroserpin antibody [C3], C-term (NBP1-31037).Red: beta Tubulin 3/ Tuj1, stained by beta Tubulin 3/ Tuj1 antibody.Blue: Fluoroshield with DAPI.

Immunohistochemistry-Frozen: Serpin I1/Neuroserpin Antibody [NBP1-31037] - Neuroserpin antibody [C3], C-term detects Neuroserpin protein by immunohistochemical analysis.Sample: Frozen-sectioned mouse cerebral cortex.Green: Neuroserpin stained by Neuroserpin antibody [C3], C-term (NBP1-31037).Blue: Fluoroshield with DAPI.

Western Blot: Serpin I1/Neuroserpin Antibody [NBP1-31037] - Sample(30 ug of whole cell lysate)A:A431 B:HeLa S3 C:Hep G2 10% SDS PAGE, antibody diluted at 1:1500.

Product Discontinued

Serpin I1/Neuroserpin Antibody Summary

• Immunogen: Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human Neuroserpin. The exact sequence is proprietary.
• Localization: Secreted
• Predicted Species: Rat (100%), Monkey (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: SERPINI1
• Purity: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence
  • Immunohistochemistry
  • Immunohistochemistry-Frozen
  • Western Blot 1:500-1:3000
• Theoretical MW: 46 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Rhesus Monkey (100%).

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7), 10% Glycerol
• Preservative: 0.01% Thimerosal
• Purity: Antigen Affinity-purified

Alternate Names for Serpin I1/Neuroserpin Antibody

• DKFZp781N13156
• Neuroserpin
• Peptidase inhibitor 12
• PI12
• PI-12
• PI12Serpin I1
• serine (or cysteine) proteinase inhibitor, clade I (neuroserpin), member 1
• Serpin I1
• serpin peptidase inhibitor, clade I (neuroserpin), member 1

Background

This gene encodes a member of the serpin superfamily of serine proteinase inhibitors. The protein is primarily secreted by axons in the brain, and preferentially reacts with and inhibits tissue-type plasminogen activator. It is thought to play a role in the regulation of axonal growth and the development of synaptic plasticity. Mutations in this gene result in familial encephalopathy with neuroserpin inclusion bodies (FENIB), which is a dominantly inherited form of familial encephalopathy and epilepsy characterized by the accumulation of mutant neuroserpin polymers. Multiple alternatively spliced variants, encoding the same protein, have been identified. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Bioinformatics

• Gene Symbol: SERPINI1