Serpin A10/ZPI Overexpression Lysate (Native)

Images

 
Western Blot: SERPINA10 Overexpression Lysate (Adult Normal) [NBP2-08261] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for SERPINA10.

Product Details

Summary
Applications WB

Order Details

Serpin A10/ZPI Overexpression Lysate (Native) Summary

Immunogen
The lysate was created in HEK293T cells, using plasmid ID RC215316 and based on accession number NP_001094077. The protein contains a C-terminal DDK tag.
Specificity
Homo sapiens SERPINA10, mRNA.
Gene
SERPINA10

Applications/Dilutions

Application Notes
This product is intended for use as a positive control in Western Blot. You will receive the lysate (100ug), and an empty vector negative control (100 ug).
Theoretical MW
50.5 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -80C. Avoid freeze-thaw cycles.
Buffer
RIPA buffer

Lysate Details for Serpin A10/ZPI

Type
Overexpression
Protein State
Native

Notes

HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.This product is manufactured by and sold under license from OriGene Technologies and its use is limited solely for research purposes.

Alternate Names for Serpin A10/ZPI Overexpression Lysate (Native)

  • antitrypsin), member 10
  • member 10
  • PZI
  • PZIprotein Z-dependent protease inhibitor
  • serine (or cysteine) proteinase inhibitor, clade A (alpha-1 antiproteinase
  • Serpin A10
  • serpin peptidase inhibitor, clade A (alpha-1 antiproteinase, antitrypsin)
  • ZPI
  • ZPIPZ-dependent protease inhibitor

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

Customers Who Viewed This Item Also Viewed...

NBP2-45405
Species: Hu
Applications: IHC
NBP1-33320
Species: Hu, Mu
Applications: WB, ICC/IF, IHC, IHC-P
NBP2-45405
Species: Hu
Applications: IHC
NBP2-34260
Species: Hu, Mu, Po, Bv, Rb
Applications: WB, Flow, ICC/IF, IHC-Fr, IHC-P, IP, PAGE
H00005555-P01
Species: Hu
NBP1-58268
Species: Hu
Applications: WB, IHC, IHC-P
AF1267
Species: Hu
Applications: WB, IP, Neut
NBP2-57614
Species: Hu
Applications: ICC/IF
NBP1-59448
Species: Hu
Applications: WB
NBP2-30949
Species: Hu
Applications: IHC, IHC-P
NBP1-81293
Species: Hu
Applications: WB, ICC/IF, IHC, IHC-P
NBP1-83408
Species: Hu
Applications: WB, ICC/IF, IHC, IHC-P
NBP1-97512
Species: Mu
Applications: WB, ICC/IF, IHC, IHC-Fr, IHC-P, IP
NBP2-49365
Species: Hu
Applications: ICC/IF, IHC
NBP1-47974
Species: Hu, Rt, Ca, Pm
Applications: WB, ICC/IF, IHC, IHC-P
NBP1-88114
Species: Hu
Applications: ICC/IF, IHC, IHC-P
NB600-586
Species: Hu
Applications: ICC/IF, IHC, IHC-P

Publications for Serpin A10/ZPI Lysate (NBP2-08261) (0)

There are no publications for Serpin A10/ZPI Lysate (NBP2-08261).
By submitting your publication information earn gift cards and discounts for future purchases.

Reviews for Serpin A10/ZPI Lysate (NBP2-08261) (0)

There are no reviews for Serpin A10/ZPI Lysate (NBP2-08261). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen
  • 1st to review with an image -- $50/€35/£30/$50 CAD/¥300 Yuan/¥5000 Yen

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for Serpin A10/ZPI Lysate (NBP2-08261) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Additional Serpin A10/ZPI Products

Bioinformatics Tool for Serpin A10/ZPI Lysate (NBP2-08261)

Discover related pathways, diseases and genes to Serpin A10/ZPI Lysate (NBP2-08261). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for Serpin A10/ZPI Lysate (NBP2-08261)

Discover more about diseases related to Serpin A10/ZPI Lysate (NBP2-08261).
 

Pathways for Serpin A10/ZPI Lysate (NBP2-08261)

View related products by pathway.

PTMs for Serpin A10/ZPI Lysate (NBP2-08261)

Learn more about PTMs related to Serpin A10/ZPI Lysate (NBP2-08261).

Blogs on Serpin A10/ZPI

There are no specific blogs for Serpin A10/ZPI, but you can read our latest blog posts.

Customers Who Bought This Also Bought

Contact Information

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our Serpin A10/ZPI Overexpression Lysate (Native) and receive a gift card or discount.

Bioinformatics

Gene Symbol SERPINA10
Entrez