Serpin A10/ZPI Antibody

Western Blot: SERPINA10 Antibody [NB100-93470] - Analysis of SERPINA10 in Human Liver lysate (35ug protein in RIPA buffer) using this antibody at 0.5 ug/ml. Primary incubation was 1 hour. Detected by chemiluminescence.

Product Discontinued

Serpin A10/ZPI Antibody Summary

• Immunogen: Peptide with sequence QSPETPAPQNQTSR-C corresponding to N-Terminus (near) according to NP_001094077.1, NP_057270.1.
• Epitope: QSPETPAPQNQTSR-C
• Specificity: This antibody is expected to recognize the isoforms (NP_001094077.1; NP_057270.1) that are shwoing 100% sequence identity.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: SERPINA10
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Peptide ELISA Detection limit 1:16000
  • Western Blot 0.5 - 1.5 ug/ml
• Application Notes: WB: Approx. 65 kDa band observed in human liver lysates is reported in scientific literature (PMID: 11049983).
• Control:

  Serpin A10/ZPI Overexpression Lysate

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for Serpin A10/ZPI Antibody

• antitrypsin), member 10
• member 10
• PZI
• PZIprotein Z-dependent protease inhibitor
• serine (or cysteine) proteinase inhibitor, clade A (alpha-1 antiproteinase
• Serpin A10
• serpin peptidase inhibitor, clade A (alpha-1 antiproteinase, antitrypsin)
• ZPI
• ZPIPZ-dependent protease inhibitor

Background

SERPINA10 inhibits factor Xa activity in the presence of protein Z, calcium and phospholipid

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Serpin A10/ZPI Antibody (NB100-93470)(2)

Publications using NB100-93470

• Corral J, Gonzalez-Conejero R, Soria JM et al. A nonsense polymorphism in the protein Z-dependent protease inhibitor increases the risk for venous thrombosis. Blood 2006-07-01 [PMID: 16527896]
• Han X, Fiehler R, Broze GJ Jr. Characterization of the protein Z-dependent protease inhibitor. Blood 2000-11-01 [PMID: 11049983]

Bioinformatics

• Gene Symbol: SERPINA10
• Entrez:
  • 51156(Human)
• Uniprot:
  • NP_001094077.1(Human)