SERCA1 ATPase Antibody

Western Blot: SERCA1 ATPase Antibody [NBP2-76912] - Western blot analysis of ATP2A1 on different lysates using anti-ATP2A1 antibody at 1/500 dilution.Positive control:Lane1: Mouse skeletal muscle tissue Lane2: Human skeletal muscle tissue

Immunohistochemistry: SERCA1 ATPase Antibody [NBP2-76912] - Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue using anti-ATP2A1 antibody. Counter stained with hematoxylin.

Flow Cytometry: SERCA1 ATPase Antibody [NBP2-76912] - Flow cytometric analysis of 293T cells with ATP2A1 antibody at 1/100 dilution (fuchsia) compared with an unlabelled control (cells without incubation with primary antibody; yellow). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.

Immunohistochemistry: SERCA1 ATPase Antibody [NBP2-76912] - Immunohistochemical analysis of paraffin-embedded rat skeletal muscle tissue using anti-ATP2A1 antibody. Counter stained with hematoxylin.

Immunohistochemistry: SERCA1 ATPase Antibody [NBP2-76912] - Immunohistochemical analysis of paraffin-embedded human fetal skeletal muscle tissue using anti-ATP2A1 antibody. Counter stained with hematoxylin.

Product Discontinued

SERCA1 ATPase Antibody Summary

• Immunogen: Synthetis peptide within mouse SERCA1 ATPase aa 1-50 / 994. (SwissProt: O14983 Human; SwissProt: Q8R429 Mouse; SwissProt: Q64578 Rat)
• Localization: Endoplasmic reticulum membrane. Sarcoplasmic reticulum membrane.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: ATP2A1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 1:50-1:100
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1:50-1:200
  • Western Blot 1:500-1:1000
• Theoretical MW: 110 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH7.4), 0.2% BSA, 50% Glycerol
• Preservative: 0.05% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for SERCA1 ATPase Antibody

• ATP2A
• ATP2A1
• ATPase, Ca++ transporting, cardiac muscle, fast twitch 1
• Calcium pump 1
• Calcium-transporting ATPase sarcoplasmic reticulum type, fast twitch skeletalmuscle isoform
• EC 3.6.3
• EC 7.2.2.10
• Endoplasmic reticulum class 1/2 Ca(2+) ATPase
• sarcoplasmic/endoplasmic reticulum calcium ATPase 1
• SERCA1 ATPase
• SERCA1
• SERCA1EC 3.6.3.8
• SR Ca(2+)-ATPase 1

Background

SERCA1 ATPase encodes one of the SERCA Ca(2+)-ATPases, which are intracellular pumps located in the sarcoplasmic or endoplasmic reticula of muscle cells. This enzyme catalyzes the hydrolysis of ATP coupled with the translocation of calcium from the cytosol to the sarcoplasmic reticulum lumen, and is involved in muscular excitation and contraction. Mutations in this gene cause some autosomal recessive forms of Brody disease, characterized by increasing impairment of muscular relaxation during exercise. Alternative splicing results in two transcript variants encoding different isoforms.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: ATP2A1