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SERCA1 ATPase Antibody

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Western Blot: SERCA1 ATPase Antibody [NBP2-76912] - Western blot analysis of ATP2A1 on different lysates using anti-ATP2A1 antibody at 1/500 dilution.Positive control:Lane1: Mouse skeletal muscle tissue Lane2: Human skeletal muscle tissue
Western Blot: SERCA1 ATPase Antibody [NBP2-76912] - Western blot analysis of ATP2A1 on different lysates using anti-ATP2A1 antibody at 1/500 dilution.Positive control:Lane1: Mouse skeletal muscle tissue Lane2: Human ...read more
Immunohistochemistry: SERCA1 ATPase Antibody [NBP2-76912] - Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue using anti-ATP2A1 antibody. Counter stained with hematoxylin.
Flow Cytometry: SERCA1 ATPase Antibody [NBP2-76912] - Flow cytometric analysis of 293T cells with ATP2A1 antibody at 1/100 dilution (fuchsia) compared with an unlabelled control (cells without incubation with primary ...read more
Immunohistochemistry: SERCA1 ATPase Antibody [NBP2-76912] - Immunohistochemical analysis of paraffin-embedded rat skeletal muscle tissue using anti-ATP2A1 antibody. Counter stained with hematoxylin.
Immunohistochemistry: SERCA1 ATPase Antibody [NBP2-76912] - Immunohistochemical analysis of paraffin-embedded human fetal skeletal muscle tissue using anti-ATP2A1 antibody. Counter stained with hematoxylin.

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, Flow, IHC, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1 mg/ml

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SERCA1 ATPase Antibody Summary

Immunogen
Synthetis peptide within mouse SERCA1 ATPase aa 1-50 / 994. (SwissProt: O14983 Human; SwissProt: Q8R429 Mouse; SwissProt: Q64578 Rat)
Localization
Endoplasmic reticulum membrane. Sarcoplasmic reticulum membrane.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
ATP2A1
Purity
Immunogen affinity purified
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Applications/Dilutions

Dilutions
  • Flow Cytometry 1:50-1:100
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1:50-1:200
  • Western Blot 1:500-1:1000
Theoretical MW
110 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH7.4), 0.2% BSA, 50% Glycerol
Preservative
0.05% Sodium Azide
Concentration
1 mg/ml
Purity
Immunogen affinity purified

Alternate Names for SERCA1 ATPase Antibody

  • ATP2A
  • ATP2A1
  • ATPase, Ca++ transporting, cardiac muscle, fast twitch 1
  • Calcium pump 1
  • Calcium-transporting ATPase sarcoplasmic reticulum type, fast twitch skeletalmuscle isoform
  • EC 3.6.3
  • EC 7.2.2.10
  • Endoplasmic reticulum class 1/2 Ca(2+) ATPase
  • sarcoplasmic/endoplasmic reticulum calcium ATPase 1
  • SERCA1 ATPase
  • SERCA1
  • SERCA1EC 3.6.3.8
  • SR Ca(2+)-ATPase 1

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Immunocytochemistry/Immunofluorescence: SERCA2 ATPase Antibody (2A7-A1) [NB300-581] - SERCA2 ATPase staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with or an antibody recognizing SERCA2 ATPase at a dilution of 1:100-1:200 over night at 4C, washed with PBS and incubated with a DyLight-488 conjugated.
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Bioinformatics

Gene Symbol ATP2A1