SERCA1 ATPase Antibody (VE121G9)

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Western Blot: SERCA1 ATPase Antibody (VE121G9) [NB300-622] - Analysis of 25 ug of human skeletal muscle (lane 1), rat skeletal muscle (lane 2) and mouse skeletal muscle (lane 3).
Immunocytochemistry/ Immunofluorescence: SERCA1 ATPase Antibody (VE121G9) [NB300-622] - SERCA1 ATPase staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Cells were grown on ...read more
Immunohistochemistry-Paraffin: SERCA1 ATPase Antibody (VE121G9) [NB300-622] - Both normal and cancer biopsies of deparaffinized Human heart tissue tissues.
Immunocytochemistry/ Immunofluorescence: SERCA1 ATPase Antibody (VE121G9) [NB300-622] - SERCA1 ATPase staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Cells were grown on ...read more
Immunocytochemistry/ Immunofluorescence: SERCA1 ATPase Antibody (VE121G9) [NB300-622] - SERCA1 ATPase staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Cells were grown on ...read more
Immunohistochemistry-Paraffin: SERCA1 ATPase Antibody (VE121G9) [NB300-622] - Both normal and cancer biopsies of deparaffinized Human skeletal muscle tissues.

Product Details

Summary
Reactivity Hu, Mu, Rt, Am, Ca, RbSpecies Glossary
Applications WB, ICC/IF, IHC, IHC-Fr, IHC-P
Clone
VE121G9
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

Order Details

SERCA1 ATPase Antibody (VE121G9) Summary

Immunogen
Purified rabbit skeletal muscle sarcoplasmic reticulum.
Epitope
This antibody recognizes an epitope between amino acid residues 506 and the C-terminus of rabbit skeletal muscle ATPase, a region that is exposed in native sarcoplasmic reticulum.
Specificity
Detects Sarcoplasmic or Endoplasmic Reticulum Calcium 1 (SERCA 1) ATPase.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
ATP2A1
Purity
Unpurified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry/Immunofluorescence 1:500
  • Immunohistochemistry 1:20
  • Immunohistochemistry-Frozen 1:10 - 1:500
  • Immunohistochemistry-Paraffin 1:10 - 1:500
  • Western Blot 1:500 - 1:5000
Application Notes
WB: Detects an approx. 110 kDa protein representing SERCA 1 ATPase in mouse muscle extracts. IF: Staining of SERCA 1 ATPase in canine skeletal muscle results in strong labeling of the entire type II (fast) myofiber. This antibody has also been shown to inhibit the crystallization of SERCA ATPase induced by vanadate.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
Ascites diluted with PBS
Preservative
0.05% Sodium Azide
Purity
Unpurified

Alternate Names for SERCA1 ATPase Antibody (VE121G9)

  • ATP2A
  • ATPase, Ca++ transporting, cardiac muscle, fast twitch 1
  • Calcium pump 1
  • Calcium-transporting ATPase sarcoplasmic reticulum type, fast twitch skeletalmuscle isoform
  • EC 3.6.3
  • Endoplasmic reticulum class 1/2 Ca(2+) ATPase
  • sarcoplasmic/endoplasmic reticulum calcium ATPase 1
  • SERCA1EC 3.6.3.8
  • SR Ca(2+)-ATPase 1

Background

ATP dependent calcium pumps are responsible, in part, for the maintenance of low cytoplasmic free calcium concentrations. The ATP pumps that reside in intracellular organelles are encoded by a family of structurally related enzymes, termed the sarcoplasmic or endoplasmic reticulum calcium (SERCA) ATPases. The SERCA 1 gene is exclusively expressed in type II (fast) skeletal muscle. The SERCA 2 gene is subject to tissue dependent processing which is responsible for the generation of SERCA 2a muscle-specific form expressed in type I (slow) skeletal, cardiac and smooth muscle and the SERCA 2b isoform expressed in all cell types. The SERCA 3 gene is not as well characterized and is found in non-muscle cells.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

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Bioinformatics

Gene Symbol ATP2A1
Entrez