SERCA1 ATPase Antibody (VE121G9)

Western Blot: SERCA1 ATPase Antibody (VE121G9) [NB300-622] - Analysis of 25 ug of human skeletal muscle (lane 1), rat skeletal muscle (lane 2) and mouse skeletal muscle (lane 3).

Immunocytochemistry/Immunofluorescence: SERCA1 ATPase Antibody (VE121G9) [NB300-622] - SERCA1 ATPase staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with or an antibody recognizing SERCA1 ATPase at a dilution of 1:20 over night at 4C, washed with PBS and incubated with a DyLight-488 conjugated.

Immunohistochemistry-Paraffin: SERCA1 ATPase Antibody (VE121G9) [NB300-622] - Both normal and cancer biopsies of deparaffinized Human heart tissue tissues.

Immunocytochemistry/Immunofluorescence: SERCA1 ATPase Antibody (VE121G9) [NB300-622] - SERCA1 ATPase staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with or an antibody recognizing SERCA1 ATPase at a dilution of 1:20 over night at 4C, washed with PBS and incubated with a DyLight-488 conjugated.

Immunocytochemistry/Immunofluorescence: SERCA1 ATPase Antibody (VE121G9) [NB300-622] - SERCA1 ATPase staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with or an antibody recognizing SERCA1 ATPase at a dilution of 1:20 over night at 4C, washed with PBS and incubated with a DyLight-488 conjugated.

Immunohistochemistry-Paraffin: SERCA1 ATPase Antibody (VE121G9) [NB300-622] - Both normal and cancer biopsies of deparaffinized Human skeletal muscle tissues.

• Reactivity: Hu, Mu, Rt, Am, Ca, Rb
• Applications: WB, ICC/IF, IHC, IHC-Fr, IHC-P
• Clone: VE121G9
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated

SERCA1 ATPase Antibody (VE121G9) Summary

• Immunogen: Purified rabbit skeletal muscle sarcoplasmic reticulum.
• Epitope: This antibody recognizes an epitope between amino acid residues 506 and the C-terminus of rabbit skeletal muscle ATPase, a region that is exposed in native sarcoplasmic reticulum.
• Specificity: Detects Sarcoplasmic or Endoplasmic Reticulum Calcium 1 (SERCA 1) ATPase.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: ATP2A1
• Purity: Unpurified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/Immunofluorescence 1:500
  • Immunohistochemistry 1:20
  • Immunohistochemistry-Frozen 1:10 - 1:500
  • Immunohistochemistry-Paraffin 1:10 - 1:500
  • Western Blot 1:500 - 1:5000
• Application Notes: WB: Detects an approx. 110 kDa protein representing SERCA 1 ATPase in mouse muscle extracts. IF: Staining of SERCA 1 ATPase in canine skeletal muscle results in strong labeling of the entire type II (fast) myofiber. This antibody has also been shown to inhibit the crystallization of SERCA ATPase induced by vanadate.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Ascites diluted with PBS
• Preservative: 0.05% Sodium Azide
• Purity: Unpurified

Alternate Names for SERCA1 ATPase Antibody (VE121G9)

• ATP2A
• ATPase, Ca++ transporting, cardiac muscle, fast twitch 1
• Calcium pump 1
• Calcium-transporting ATPase sarcoplasmic reticulum type, fast twitch skeletalmuscle isoform
• EC 3.6.3
• Endoplasmic reticulum class 1/2 Ca(2+) ATPase
• sarcoplasmic/endoplasmic reticulum calcium ATPase 1
• SERCA1EC 3.6.3.8
• SR Ca(2+)-ATPase 1

Background

ATP dependent calcium pumps are responsible, in part, for the maintenance of low cytoplasmic free calcium concentrations. The ATP pumps that reside in intracellular organelles are encoded by a family of structurally related enzymes, termed the sarcoplasmic or endoplasmic reticulum calcium (SERCA) ATPases. The SERCA 1 gene is exclusively expressed in type II (fast) skeletal muscle. The SERCA 2 gene is subject to tissue dependent processing which is responsible for the generation of SERCA 2a muscle-specific form expressed in type I (slow) skeletal, cardiac and smooth muscle and the SERCA 2b isoform expressed in all cell types. The SERCA 3 gene is not as well characterized and is found in non-muscle cells.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: ATP2A1
• Entrez:
  • 11937(Mouse)