Septin-8 Antibody

Images

 
Western Blot: Septin-8 Antibody [NBP2-15116] - Various tissue extracts (10 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with Septin-8 antibody diluted at 1:4000. HRP-conjugated anti-rabbit IgG ...read more
Immunocytochemistry/ Immunofluorescence: Septin-8 Antibody [NBP2-15116] - Septin-8 antibody detects Septin-8 protein by immunofluorescent analysis. Sample: DIV9 rat E18 primary cortical neurons were fixed in 4% ...read more
Immunohistochemistry-Paraffin: Septin-8 Antibody [NBP2-15116] - Paraffin-embedded U87 xenograft; antibody dilution 1:500.
Western Blot: Septin-8 Antibody [NBP2-15116] - Sample (30 ug of whole cell lysate) A: HCT116 12% SDS PAGE, antibody diluted at 1:2000.
Immunocytochemistry/ Immunofluorescence: Septin-8 Antibody [NBP2-15116] - Analysis of methanol-fixed HeLa, using 39332 antibody (Green) at 1:200 dilution. Alpha-tubulin filaments were labeled with an alpha Tubulin ...read more

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, ICC/IF, IHC
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

Septin-8 Antibody Summary

Immunogen
Recombinant protein encompassing a sequence within the center region of human SEPT8. The exact sequence is proprietary.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
SEPTIN8
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence 1:100-1:1000
  • Immunohistochemistry 10 - 1:500
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • Western Blot 1:500-1:10000

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
0.1M Tris (pH 7), 0.1M Glycine, 20% Glycerol
Preservative
0.01% Thimerosal
Purity
Immunogen affinity purified

Alternate Names for Septin-8 Antibody

  • KIAA0202SEP2
  • septin 8
  • septin-8

Background

SEPT8 is a member of the highly conserved septin family. Septins are 40- to 60-kD GTPases that assemble as filamentous scaffolds. They are involved in the organization of submembranous structures, in neuronal polarity, and in vesicle trafficking (Blaser et al., 2003 [PubMed 12909369]).[supplied by OMIM]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Bioinformatics

Gene Symbol SEPTIN8
Uniprot