Septin-7 Overexpression Lysate

Images

 
Western Blot: Septin-7 Overexpression Lysate (Adult Normal) [NBL1-07149] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for Septin-7.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB

Order Details

Septin-7 Overexpression Lysate Summary

Description
Transient overexpression lysate of septin 7 (SEPT7), transcript variant 1 The lysate was created in HEK293T cells, using Plasmid ID RC205163 and based on accession number NM_001788. The protein contains a C-MYC/DDK Tag.
Gene
SEPTIN7

Applications/Dilutions

Dilutions
  • Western Blot
Application Notes
This product is intended for use as a positive control in Western Blot.

You will receive 1 vial of lysate (100ug), 1 vial of empty vector negative control (100ug), and 1 vial of 2xSDS sample buffer (250ul).
Theoretical MW
50.5 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -80C. Avoid freeze-thaw cycles.
Buffer
RIPA buffer

Lysate Details for Array

Type
Overexpression

Notes

HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.This product is manufactured by and sold under license from OriGene Technologies and its use is limited solely for research purposes.

Alternate Names for Septin-7 Overexpression Lysate

  • CDC10 cell division cycle 10 homolog (S. cerevisiae)
  • CDC10 cell division cycle 10 homolog
  • CDC10 protein homolog
  • CDC10S. cerevisiae, homolog)
  • Nbla02942
  • septin 7
  • septin-7

Background

This gene encodes a protein that is highly similar to the CDC10 protein of Saccharomyces cerevisiae. The protein also shares similarity with Diff 6 of Drosophila and with H5 of mouse. Each of these similar proteins, including the yeast CDC10, contains a GTP-binding motif. The yeast CDC10 protein is a structural component of the 10 nm filament which lies inside the cytoplasmic membrane and is essential for cytokinesis. Although the exact function of this gene has not yet been determined, its high similarity to yeast CDC10 and the high conservative nature of eukaryotic cell cycle machinery suggest a similar role to that of its yeast counterpart. Alternative splicing results in two transcript variants encoding different isoforms. [provided by RefSeq]. Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

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Publications for Septin-7 Lysate (NBL1-07149) (0)

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Bioinformatics

Gene Symbol SEPTIN7