Septin-7 Antibody

Western Blot: Septin-7 Antibody [NBP1-06077] - Staining of nuclear HeLa lysate (35 ug protein in RIPA buffer). Antibody at 0.5 ug/mL. Primary incubation was 1 hour. Detected by chemiluminescence.

• Reactivity: Hu, Mu, Rt, Po, Bv, Ca, Eq
• Applications: WB, ELISA
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: 0.5 mg/ml

Septin-7 Antibody Summary

• Immunogen: Peptide with sequence C-DNNKNKGQLTKSP corresponding to internal region according to NP_001011553.2, NP_001229885.1.
• Epitope: C-DNNKNKGQLTKSP
• Specificity: This antibody is expected to recognize all three reported isoforms (NP_001779.3; NP_001011553.2; NP_001229885.1).
• Predicted Species: Mouse (100%), Rat (100%), Porcine (100%), Bovine (100%), Canine (100%), Equine (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: SEPTIN7
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Peptide ELISA Detection limit 1:32000
  • Western Blot 0.3 - 1 ug/mL
• Application Notes: WB: Approx. 45 kDa band observed in HeLa nuclear lysates (calculated MW of 50.7 kDa band according to NP_001779.3 and of 55.6 kDa band according to NP_001011553.2). The observed molecular weight corresponds to earlier findings with different antibodies from other sources.
• Control:

  Septin-7 Overexpression Lysate

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for Septin-7 Antibody

• CDC10 cell division cycle 10 homolog (S. cerevisiae)
• CDC10 cell division cycle 10 homolog
• CDC10 protein homolog
• CDC10S. cerevisiae, homolog)
• Nbla02942
• septin 7
• septin-7

Background

This gene encodes a protein that is highly similar to the CDC10 protein of Saccharomyces cerevisiae. The protein also shares similarity with Diff 6 of Drosophila and with H5 of mouse. Each of these similar proteins, including the yeast CDC10, contains a GTP-binding motif. The yeast CDC10 protein is a structural component of the 10 nm filament which lies inside the cytoplasmic membrane and is essential for cytokinesis. Although the exact function of this gene has not yet been determined, its high similarity to yeast CDC10 and the high conservative nature of eukaryotic cell cycle machinery suggest a similar role to that of its yeast counterpart. Alternative splicing results in two transcript variants encoding different isoforms. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: SEPTIN7
• Entrez:
  • 989(Human)
  • 235072(Mouse)
  • 64551(Rat)
• Uniprot:
  • NP_001779.3(Human)