Septin-6 Antibody

Western Blot: Septin-6 Antibody [NBP1-51930] - Staining (1ug/ml) of Human Testis (A), (0.1ug/ml) Duodenum (B), (0.5ug/ml) Kidney (C) and (0.3ug/ml) Tonsil (D) lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.

Immunohistochemistry-Paraffin: Septin-6 Antibody [NBP1-51930] - Staining of paraffin embedded Human Tonsil. Antibody at 3.8 ug/mL. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Western Blot: Septin-6 Antibody [NBP1-51930] - Staining (0.1ug/ml) of Daudi (A), Jurkat (B) and MOLT4 (C) cell lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.

• Reactivity: Hu, Bv, Ca
• Applications: WB, ELISA, IHC
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: 0.5 mg/ml

Septin-6 Antibody Summary

• Description: Novus Biologicals Goat Septin-6 Antibody (NBP1-51930) is a polyclonal antibody validated for use in IHC, WB and ELISA. Anti-Septin-6 Antibody: Cited in 1 publication. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Peptide with sequence C-DEVNAFKQRKTA corresponding to internal region according to NP_665798.1, NP_055944.2, NP_665801.1.
• Epitope: C-DEVNAFKQRKTA
• Localization: In paraffin embedded Human Tonsil shows specific staining in dividing cells just outside a germinal centre
• Specificity: This antibody is expected to recognize the reported isoforms NP_665798.1, NP_055944.2 and NP_665801.1. Reported variants NP_665799.1 and NP_665798.1 represent identical protein.
• Predicted Species: Canine (100%), Bovine (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: SEPTIN6
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 3 - 5 ug/mL
  • Immunohistochemistry-Paraffin 3 - 5 ug/mL
  • Peptide ELISA Detection limit 1:4000
  • Western Blot 0.1 - 1 ug/mL
• Application Notes: WB: Approx 50 kDa band observed in MOLT4, Daudi, Jurkat, Human Testis, Duodenum, Kidney and Tonsil lysates (calculated MW of 49.7 kDa according to NP_055944.2).

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for Septin-6 Antibody

• KIAA0128SEP2septin 2
• MGC16619
• MGC20339
• SEPT2
• septin 6
• septin-6

Background

This gene is a member of the septin family of GTPases. Members of this family are required for cytokinesis. One version of pediatric acute myeloid leukemia is the result of a reciprocal translocation between chromosomes 11 and X, with the breakpoint associated with the genes encoding the mixed-lineage leukemia and septin 2 proteins. This gene encodes four transcript variants encoding three distinct isoforms. An additional transcript variant has been identified, but its biological validity has not been determined. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Septin-6 Antibody (NBP1-51930)(1)

Publication using NBP1-51930

• Kremer BE, Adang LA, Macara IG. Septins regulate actin organization and cell-cycle arrest through nuclearaccumulation of NCK mediated by SOCS7. Cell 2007-09-07 [PMID: 17803907]

Bioinformatics

• Gene Symbol: SEPTIN6
• Entrez:
  • 23157(Human)
• Uniprot:
  • NP_665798.1(Human)