Semaphorin 4C Antibody (663203) [Alexa Fluor® 647]

• Reactivity: Mu
• Applications: Flow
• Clone: 663203
• Clonality: Monoclonal
• Host: Rat
• Conjugate: Alexa Fluor 647
• Concentration: Please see the vial label for concentration. If unlisted please contact technical services.

Semaphorin 4C Antibody (663203) [Alexa Fluor® 647] Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant mouse Semaphorin 4C
  Ala21-Phe664
  Accession # Q64151
• Specificity: Detects mouse Semaphorin 4C in direct ELISAs. Detects HEK293 human embryonic kidney cell line transfected with mouse Semaphorin 4C by Flow Cytometry. Does not detect untransfected or irrelevant transfected HEK293 cells.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Rat
• Gene: SEMA4C
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 0.25-1 ug/10^6 cells
• Application Notes: Flow Cytometry: Please use 0.25-1 ug of conjugated antibody per 10e6 cells.

Packaging, Storage & Formulations

• Storage: Store the unopened product at 2 - 8 °C. Do not use past expiration date.
• Buffer: Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.
• Preservative: 0.09% Sodium Azide
• Concentration: Please see the vial label for concentration. If unlisted please contact technical services.

Notes

This product is provided under an agreement between Life Technologies Corporation and R&D Systems, Inc, and the manufacture, use, sale or import of this product is subject to one or more US patents and corresponding non-US equivalents, owned by Life Technologies Corporation and its affiliates. The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components (1) in manufacturing; (2) to provide a service, information, or data to an unaffiliated third party for payment; (3) for therapeutic, diagnostic or prophylactic purposes; (4) to resell, sell, or otherwise transfer this product or its components to any third party, or for any other commercial purpose. Life Technologies Corporation will not assert a claim against the buyer of the infringement of the above patents based on the manufacture, use or sale of a commercial product developed in research by the buyer in which this product or its components was employed, provided that neither this product nor any of its components was used in the manufacture of such product. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, Cell Analysis Business Unit, Business Development, 29851 Willow Creek Road, Eugene, OR 97402, Tel: (541) 465-8300. Fax: (541) 335-0354.

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Semaphorin 4C Antibody (663203) [Alexa Fluor® 647]

• FLJ20369
• KIAA1739
• MGC126382
• MGC126383
• M-Sema F
• M-SEMA-F
• sema domain, immunoglobulin domain (Ig), transmembrane domain (TM) and shortcytoplasmic domain, (semaphorin) 4C
• SEMA4C
• SEMACL1
• Semaf
• SEMAI
• Semaphorin 4C
• semaphorin-4C

Background

Sema4C (Semaphorin 4C; also Sema I and M-SemaF) is a 100-105 kDa, class IV member of the semaphorin family of proteins. It is expressed by precursors to neurons and myocytes, and may regulate their differentiation into mature forms. Mature mouse Sema4C is a type I transmembrane glycoprotein that is 814 amino acids (aa) in length. It contains a 644 aa extracellular region (aa 21-664) that is characterized by the presence of one Sema domain (aa 30-497), a PSI region (aa 499‑552), and an Ig-like C2-type domain (aa 557-645). The cytoplasmic region interacts with PZD-domain containing proteins. Over aa 21-664, mouse Sema4C shares 85% and 95% aa identity with human and rat Sema4C, respectively.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Semaphorin 4C Antibody (FAB8497R)(1)

Publication using FAB8497R

• Borrelli, C;Roberts, M;Eletto, D;Hussherr, MD;Fazilaty, H;Valenta, T;Lafzi, A;Kretz, JA;Guido Vinzoni, E;Karakatsani, A;Adivarahan, S;Mannhart, A;Kimura, S;Meijs, A;Baccouche Mhamedi, F;Acar, IE;Handler, K;Ficht, X;Platt, RJ;Piscuoglio, S;Moor, AE; In vivo interaction screening reveals liver-derived constraints to metastasis Nature 2024-07-24 [PMID: 39048831] (Immunohistochemistry, Transgenic Mouse)

Bioinformatics

• Gene Symbol: SEMA4C
• Uniprot:
  • Q64151()