Secretin R Antibody (783526)

Western blot shows lysates of Capan-1 human pancreatic adenocarcinoma cell line and A549 human lung carcinoma cell line. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human Secretin R Monoclonal Antibody (Catalog # MAB6387) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for Secretin R at approximately 64 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Product Discontinued

Secretin R Antibody (783526) Summary

• Immunogen: E. coli-derived recombinant human Secretin R delta 3/4Splice variant (1-3)
  Gly65-Asn132
  Accession # P47872
• Specificity: Detects human Secretin R in direct ELISAs and Western Blot.
• Source: N/A
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Gene: SCTR
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Western Blot 2 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Sterile PBS to a final concentration of 0.5 mg/mL.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Secretin R Antibody (783526)

• pancreatic secretin receptor
• SCTR
• SCT-R
• Secretin R
• secretin receptor
• SecretinR
• SR

Background

SCTR (Secretin receptor) is a 51-64 kDa member of the G-protein coupled receptor 2 family. It is found on alveolar epithelium, bile duct epithelium, pancreatic exocrine duct epithelium, and stomach plus duodenal mucosal epithelium. Mature human SCTR is a 418 amino acid (aa) 7-transmembrane glycoprotein (aa 23-440). It contains a 121 aa N-terminal extracellular region (aa 23-143) plus a 48 aa C-terminal cytoplasmic domain (aa 393-440). There is at least one splice variant that shows a deletion of aa 66-101. SCTR forms homodimers and homooligomers, and heterodimerizes with almost all family B GPCRs (VPAC1; VPAC2; GLP1R; GHRHR; etc.). A splice form of the human Secretin Receptor with deletion of exons 3 and 4  was found expressed in pancreatic adenocarcinomas and cholangiocellular carcinomas, but not in gastrinomas or nonneoplastic pancreas or liver specimens.The deletion in this splice form is predicted to lead to a frame-shift, early truncation, and total absence of transmembrane segments in the receptor protein, whereas the leader sequence responsible for trafficking of the receptor to the cell membrane is preserved. This encoded a 111 aa peptide with its first 43 residues identical to wild-type receptor; but, subsequent to a shift in coding frame and early truncation, the next 68 residues were unique in the transcriptome/proteome. Our antibody was raised against this unique 68aa sequence resulting from putative frame-shift. 

1. Hayes, G.M. et al. (2007) Gastroenterology 133:853.
2. Korner, M. et al. (2005) Am. J. Pathol. 167:959.
3. Korner, M. et al. (2006) J. Hepatol. 45:825.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: SCTR
• Uniprot:
  • P47872(Human)