Recombinant Human SDNSF/MCFD2 Protein

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SDS-Page: MCFD2 Protein [NBP1-37074] - MCFD2, 15.1 kDa (136aa), confirmed by MALDI-TOF with a purity of 90% by SDS - PAGE

Product Details

Summary
Reactivity HuSpecies Glossary
Applications PAGE
Concentration
1 mg/ml

Order Details

Recombinant Human SDNSF/MCFD2 Protein Summary

Description
A recombinant protein with a N-Terminal T7-tag and corresponding to the amino acids 27-146 of Human SDNSF/MCFD2

Source: E.coli

Amino Acid Sequence: MASMTGGQQM GRGSHMEEPA ASFSQPGSMG LDKNTVHDQE HIMEHLEGVI NKPEAEMSPQ ELQLHYFKMH DYDGNNLLDG LELSTAITHV HKEEGSEQAP LMSEDELINI IDGVLRDDDK NNDGYIDYAE FAKSLQ

Source
E. coli
Protein/Peptide Type
Recombinant Protein
Gene
MCFD2
Purity
>90%, by SDS-PAGE

Applications/Dilutions

Theoretical MW
15.1 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
20mM Tris-HCl buffer (pH7.5) containing 100mM NaCl, 10% glycerol
Preservative
No Preservative
Concentration
1 mg/ml
Purity
>90%, by SDS-PAGE

Alternate Names for Recombinant Human SDNSF/MCFD2 Protein

  • DKFZp686G21263
  • F5F8D2
  • LMAN1IP
  • MCFD2
  • multiple coagulation factor deficiency 2
  • multiple coagulation factor deficiency protein 2
  • neural stem cell derived neuronal survival protein
  • Neural stem cell-derived neuronal survival protein
  • SDNSF
  • SDNSFF5F8D

Background

Multiple coagulation factor deficiency 2 (MCFD2), also known as SDNSF. This is expressed by neural stem/progenitor cells of the hippocampus, and localized to region where neurogenesis persists throughout life. It has been found to prevent NSC cell death and to maintain stem cell characteristics. This protein forms a complex with LAMN1 that facilitates the transport of coagulation factors V and VIII from the endoplasmic reticulum to the Golgi apparatus via an endoplasmic reticulum Golgi intermediate compartment. Mutations in the MCFD2 gene may cause Factor V and Factor VIII combined deficiency. Recombinant human MCFD2, fused to T7-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

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Bioinformatics

Gene Symbol MCFD2
Entrez