SDHD Antibody [Alexa Fluor™ Plus 405]

• Reactivity: Hu, Mu, Rt, Bv, Sh
• Applications: WB, ELISA, ICC/IF
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Alexa Fluor Plus 405

SDHD Antibody [Alexa Fluor™ Plus 405] Summary

• Description: This conjugate is made on demand and is not held in inventory. Each order represents a unique lot. All conjugates are generated using validated conjugation protocols and meet strict degree of labeling (F/P) specifications; additional information is available upon request. The antibody concentration is provided on the individual product label.
  
  For specialized conjugation needs, like large quantities, specific concentrations, and defined F/P ratios, bulk and custom antibody conjugation services are available.
• Immunogen: SDHD antibody was raised against a 15 amino acid synthetic peptide near the center of human SDHD. The immunogen is located within amino acids 30 - 80 of SDHD.
• Specificity: At least four isoforms of SDHD are known to exist; this antibody will detect the two longest isoforms.
• Predicted Species: Sheep (100%), Bovine (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: SDHD
• Purity: Peptide affinity purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Immunocytochemistry/ Immunofluorescence
  • Western Blot
• Application Notes: Recommended applications are based on validated applications from the unconjugated base product NBP2-81948. This conjugated antibody is not kept in inventory and is made to order using validated conjugation protocols.

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Porcine: (80%)

Packaging, Storage & Formulations

• Storage: Store at 4C in the dark.
• Buffer: 50mM Sodium Borate
• Preservative: 0.05% Sodium Azide
• Purity: Peptide affinity purified

Notes

This product is provided under an intellectual property license from Life Technologies Corporation. The transfer of this product is conditioned on the buyer using the purchased product solely in research conducted by the buyer, excluding contract research or any fee for service research, and the buyer must not (1) use this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; or (c) manufacturing or quality assurance or quality control, and/or (2) sell or transfer this product or its components for resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com. This conjugate is made on demand. Actual recovery may vary from the stated volume of this product. The volume will be greater than or equal to the unit size stated on the datasheet.

Alternate Names for SDHD Antibody [Alexa Fluor™ Plus 405]

• CBT1
• CII-4
• CybS
• PGL
• PGL1
• QPs3
• SDH4
• succinate dehydrogenase [ubiquinone] cytochrome b small subunit, mitochondrial
• Succinate dehydrogenase complex subunit D
• succinate dehydrogenase complex, subunit D, integral membrane protein
• succinate dehydrogenase ubiquinone cytochrome B small subunit
• Succinate-ubiquinone oxidoreductase cytochrome b small subunit
• Succinate-ubiquinone reductase membrane anchor subunit

Background

SDHD is part of Complex II. Complex II of the respiratory chain, which is specifically involved in the oxidation of succinate, carries electrons from FADH to CoQ. The complex is composed of four nuclear-encoded subunits and is localized in the mitochondrial inner membrane. The subunit D protein is one of two integral membrane proteins anchoring the complex to the matrix side of the membrane. Mutations in SDHD have been linked to hereditary paraganglioma.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: SDHD