SDHA Overexpression Lysate (Native)

Images

 
Western Blot: SDHA Overexpression Lysate (Adult Normal) [NBL1-15765] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for SDHA.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB

Order Details

SDHA Overexpression Lysate (Native) Summary

Immunogen
The lysate was created in HEK293T cells, using plasmid ID RC200349 and based on accession number NM_004168. The protein contains a C-terminal DDK tag.
Specificity
Homo sapiens succinate dehydrogenase complex, subunit A, flavoprotein (Fp) (SDHA), nuclear gene encoding mitochondrial protein, mRNA.
Gene
SDHA

Applications/Dilutions

Application Notes
This product is intended for use as a positive control in Western Blot. You will receive the lysate (100ug), and an empty vector negative control (100 ug).
Theoretical MW
72.5 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -80C. Avoid freeze-thaw cycles.
Buffer
RIPA buffer

Lysate Details for SDHA

Type
Overexpression
Protein State
Native

Notes

HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.This product is manufactured by and sold under license from OriGene Technologies and its use is limited solely for research purposes.

Alternate Names for SDHA Overexpression Lysate (Native)

  • CMD1GG
  • EC 1.3.5.1
  • Fp
  • SDH1
  • SDH2
  • SDHFmitochondrial
  • succinate dehydrogenase complex flavoprotein subunit
  • succinate dehydrogenase complex, subunit A, flavoprotein (Fp)

Background

This gene encodes a major catalytic subunit of succinate-ubiquinone oxidoreductase, a complex of the mitochondrial respiratory chain. The complex is composed of four nuclear-encoded subunits and is localized in the mitochondrial inner membrane. Mutations in this gene have been associated with a form of mitochondrial respiratory chain deficiency known as Leigh Syndrome. A pseudogene has been identified on chromosome 3q29. [provided by RefSeq]. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

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Publications for SDHA Lysate (NBL1-15765) (0)

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Product General Protocols

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Video Protocols

WB Video Protocol

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Blogs on SDHA.

SDHA - oxidative enzyme in the citric acid cycle
Succinate dehydrogenase is an important tetrameric protein involved in the citric acid cycle. It is localized to the inner mitochondrial membrane of cells. Succinate dehydrogenase makes up Complex II of the electron transport chain (ETC) and is re...  Read full blog post.

SDHA - An essential Krebs cycle enzyme with role in cancer and metabolism
Succinate dehydrogenase (SDH) is a highly conserved protein complex located on the inner mitochondrial membrane where it functions during the Krebs cycle by oxidizing succinate to fumarate (1). This reaction is also important for feeding electrons...  Read full blog post.

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Bioinformatics

Gene Symbol SDHA