SDHA Antibody (JM10-83)

Western Blot: SDHA Antibody (JM10-83) [NBP2-67130] - Western blot analysis of SDHA on different cell lysates using anti-SDHA antibody at 1/500 dilution. Positive control: Lane 1:HepG2 Lane 2:SHG44

Immunocytochemistry/Immunofluorescence: SDHA Antibody (JM10-83) [NBP2-67130] - Staining SDHA in NIH-3T3 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunohistochemistry-Paraffin: SDHA Antibody (JM10-83) [NBP2-67130] - Analysis of paraffin-embedded zebrafish tissue using anti-SDHA antibody. Counter stained with hematoxylin.

Flow Cytometry: SDHA Antibody (JM10-83) [NBP2-67130] - Flow cytometric analysis of Hela cells with SDHA antibody at 1/50 dilution (red) compared with an unlabeled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.

Western Blot: SDHA Antibody (JM10-83) [NBP2-67130] - Analysis of SDHA on Jurkat cells lysates using anti-SDHA antibody at 1/500 dilution.

Immunocytochemistry/Immunofluorescence: SDHA Antibody (JM10-83) [NBP2-67130] - Staining SDHA in HepG2 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunocytochemistry/Immunofluorescence: SDHA Antibody (JM10-83) [NBP2-67130] - Staining SDHA in MCF-7 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunohistochemistry-Paraffin: SDHA Antibody (JM10-83) [NBP2-67130] - Analysis of paraffin-embedded human kidney tissue using anti-SDHA antibody. Counter stained with hematoxylin.

Immunohistochemistry-Paraffin: SDHA Antibody (JM10-83) [NBP2-67130] - Analysis of paraffin-embedded human liver tissue using anti-SDHA antibody. Counter stained with hematoxylin.

Immunohistochemistry-Paraffin: SDHA Antibody (JM10-83) [NBP2-67130] - Analysis of paraffin-embedded mouse colon tissue using anti-SDHA antibody. Counter stained with hematoxylin.

Immunohistochemistry-Paraffin: SDHA Antibody (JM10-83) [NBP2-67130] - Analysis of paraffin-embedded mouse skeletal muscle tissue using anti-SDHA antibody. Counter stained with hematoxylin.

Immunohistochemistry-Paraffin: SDHA Antibody (JM10-83) [NBP2-67130] - Analysis of paraffin-embedded mouse testis tissue using anti-SDHA antibody. Counter stained with hematoxylin.

Product Discontinued

SDHA Antibody (JM10-83) Summary

• Additional Information: Recombinant Monoclonal Antibody.
• Immunogen: Synthetic peptide within Human SDHA aa 581-624 / 664. (SwissProt: P31040 Human; SwissProt: Q8K2B3 Mouse; SwissProt: Q920L2 Rat)
• Localization: Membrane, Mitochondrion, Mitochondrion inner membrane.
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: SDHA
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 1:50-1:100
  • Immunocytochemistry/ Immunofluorescence 1:50-1:200
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1:50-1:5000
  • Western Blot 1:500-1:1000

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: TBS (pH7.4), 0.05% BSA, 40% Glycerol
• Preservative: 0.05% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein A purified

Alternate Names for SDHA Antibody (JM10-83)

• CMD1GG
• flavoprotein subunit of complex II
• FP
• MC2DN1
• NDAXOA
• PGL5
• PPGL5
• SDH1
• SDH2
• SDHA
• SDHF
• succinate dehydrogenase complex, subunit A, flavoprotein (Fp)

Background

The SDHA gene encodes a major catalytic subunit of succinate-ubiquinone oxidoreductase, a complex of the mitochondrial respiratory chain. The complex is composed of four nuclear-encoded subunits and is localized in the mitochondrial inner membrane. Mutations

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Review for SDHA Antibody (NBP2-67130) (1)

Average Rating: 5

(Based on 1 review)

We have 1 review tested in 1 species: Mouse.

reviewed by: zhenqi zhou WB Mouse 03/12/2019

Summary

• Application: Western Blot
• Sample Tested: c2c12
• Species: Mouse
• Lot: hk0213

Bioinformatics

• Gene Symbol: SDHA