![Western Blot: SCY1 like 3 Antibody (OTI1A5) - Azide and BSA Free [NBP2-74025] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY SCY1 like 3 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-SCY1 like](http://images.novusbio.com/fullsize/SCY1-like-3-Antibody-OTI1A5-Azide-and-BSA-Free-Western-Blot-NBP2-74025-img0001.jpg "Western Blot: SCY1 like 3 Antibody (OTI1A5) - Azide and BSA Free [NBP2-74025] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY SCY1 like 3 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-SCY1 like")
| Reactivity | HuSpecies Glossary |
| Applications | WB, IHC |
| Clone | OTI1A5 |
| Clonality | Monoclonal |
| Host | Mouse |
| Conjugate | Unconjugated |
| Format | Azide and BSA Free |
| Concentration | LYOPH |
| Description | Novus Biologicals Mouse SCY1 like 3 Antibody (OTI1A5) - Azide and BSA Free (NBP1-47956) is a monoclonal antibody validated for use in IHC and WB. All Novus Biologicals antibodies are covered by our 100% guarantee. |
| Immunogen | Full-length protein expressed in 293T cell transfected with human SCYL3 expression vector |
| Isotype | IgG1 |
| Clonality | Monoclonal |
| Host | Mouse |
| Gene | SCYL3 |
| Purity | Immunogen affinity purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Theoretical MW | 82.8 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
| Storage | Store at -20C. Avoid freeze-thaw cycles. |
| Buffer | Lyophilized from PBS (pH 7.3) with 8% Trehalose |
| Preservative | No Preservative |
| Concentration | LYOPH |
| Purity | Immunogen affinity purified |
| Reconstitution Instructions | we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. |
Secondary Antibodies |
Isotype Controls |
The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.
| Gene Symbol | SCYL3 |
![Immunohistochemistry: SCY1 like 3 Antibody (OTI1A5) - Azide and BSA Free [NBP2-74025] - Staining of paraffin-embedded endometrium tissue using anti-SCY1 like 3mouse monoclonal antibody.](http://images.novusbio.com/fullsize/SCY1-like-3-Antibody-OTI1A5-Azide-and-BSA-Free-Immunohistochemistry-NBP2-74025-img0007.jpg "Immunohistochemistry: SCY1 like 3 Antibody (OTI1A5) - Azide and BSA Free [NBP2-74025] - Staining of paraffin-embedded endometrium tissue using anti-SCY1 like 3mouse monoclonal antibody.")
![Immunohistochemistry: SCY1 like 3 Antibody (OTI1A5) - Azide and BSA Free [NBP2-74025] - Staining of paraffin-embedded Adenocarcinoma of breast tissue using anti-SCY1 like 3 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/SCY1-like-3-Antibody-OTI1A5-Azide-and-BSA-Free-Immunohistochemistry-NBP2-74025-img0002.jpg "Immunohistochemistry: SCY1 like 3 Antibody (OTI1A5) - Azide and BSA Free [NBP2-74025] - Staining of paraffin-embedded Adenocarcinoma of breast tissue using anti-SCY1 like 3 mouse monoclonal antibody.")
![Immunohistochemistry: SCY1 like 3 Antibody (OTI1A5) - Azide and BSA Free [NBP2-74025] - Staining of paraffin-embedded Adenocarcinoma of colon tissue using anti-SCY1 like 3 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/SCY1-like-3-Antibody-OTI1A5-Azide-and-BSA-Free-Immunohistochemistry-NBP2-74025-img0003.jpg "Immunohistochemistry: SCY1 like 3 Antibody (OTI1A5) - Azide and BSA Free [NBP2-74025] - Staining of paraffin-embedded Adenocarcinoma of colon tissue using anti-SCY1 like 3 mouse monoclonal antibody.")
![Immunohistochemistry: SCY1 like 3 Antibody (OTI1A5) - Azide and BSA Free [NBP2-74025] - Staining of paraffin-embedded breast tissue using anti-SCY1 like 3 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/SCY1-like-3-Antibody-OTI1A5-Azide-and-BSA-Free-Immunohistochemistry-NBP2-74025-img0004.jpg "Immunohistochemistry: SCY1 like 3 Antibody (OTI1A5) - Azide and BSA Free [NBP2-74025] - Staining of paraffin-embedded breast tissue using anti-SCY1 like 3 mouse monoclonal antibody.")
![Immunohistochemistry: SCY1 like 3 Antibody (OTI1A5) - Azide and BSA Free [NBP2-74025] - Staining of paraffin-embedded Carcinoma of kidney tissue using anti-SCY1 like 3 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/SCY1-like-3-Antibody-OTI1A5-Azide-and-BSA-Free-Immunohistochemistry-NBP2-74025-img0005.jpg "Immunohistochemistry: SCY1 like 3 Antibody (OTI1A5) - Azide and BSA Free [NBP2-74025] - Staining of paraffin-embedded Carcinoma of kidney tissue using anti-SCY1 like 3 mouse monoclonal antibody.")
![Immunohistochemistry: SCY1 like 3 Antibody (OTI1A5) - Azide and BSA Free [NBP2-74025] - Staining of paraffin-embedded Carcinoma of thyroid tissue using anti-SCY1 like 3 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/SCY1-like-3-Antibody-OTI1A5-Azide-and-BSA-Free-Immunohistochemistry-NBP2-74025-img0006.jpg "Immunohistochemistry: SCY1 like 3 Antibody (OTI1A5) - Azide and BSA Free [NBP2-74025] - Staining of paraffin-embedded Carcinoma of thyroid tissue using anti-SCY1 like 3 mouse monoclonal antibody.")
![Immunohistochemistry: SCY1 like 3 Antibody (OTI1A5) - Azide and BSA Free [NBP2-74025] - Staining of paraffin-embedded pancreas tissue using anti-SCY1 like 3mouse monoclonal antibody.](http://images.novusbio.com/fullsize/SCY1-like-3-Antibody-OTI1A5-Azide-and-BSA-Free-Immunohistochemistry-NBP2-74025-img0008.jpg "Immunohistochemistry: SCY1 like 3 Antibody (OTI1A5) - Azide and BSA Free [NBP2-74025] - Staining of paraffin-embedded pancreas tissue using anti-SCY1 like 3mouse monoclonal antibody.")
![SDS-Page: Mouse IgG1 Isotype Control (MG1) [NBP1-97005] - Lane 1: , Non-reduced. M: Opal Pre-stained Ladder. Lane 2: , Reduced. Load: 1.0 ug per lane. Predicted/Observed: 120 kDa Non-reduced, 55 and 25 Reduced.](https://images.novusbio.com/images/Mouse-IgG1-Isotype-Control-MG1-SDS-Page-NBP1-97005-img0002.jpg "SDS-Page: Mouse IgG1 Isotype Control (MG1) [NBP1-97005] - Lane 1: , Non-reduced. M: Opal Pre-stained Ladder. Lane 2: , Reduced. Load: 1.0 ug per lane. Predicted/Observed: 120 kDa Non-reduced, 55 and 25 Reduced.")