Scn1a Antibody (K74/71) - Chimeric - Azide and BSA Free

Representative section of formalin fixed, paraffin-embedded rat brain cerebellum region showing cells stained by NBP3-52336 (the BSA Free version of this antibody) using goat anti-chicken IgY secondary antibody (visualized in green). DAPI nuclear stain (blue) shows cell nuclei.

Western blotting of rat brain membrane with NBP3-52336 (the BSA Free version of this antibody). The chicken mAb was detected with anti-chicken HRP. This antibody recognizes endogenous Scn1a in the rat brain membrane fraction at ~220 kDa.

Representative section of formalin fixed, paraffin-embedded rat brain hippocampal CA2/CA3 region showing cells stained by NBP3-52336 (the BSA Free version of this antibody) using goat anti-chicken IgY secondary antibody (visualized in green). DAPI nuclear stain (blue) shows cell nuclei.

• Reactivity: Hu, Mu, Rt, Pm
• Applications: WB, ELISA, ICC/IF, IHC, IP
• Clone: K74/71
• Clonality: Monoclonal
• Host: Chicken
• Conjugate: Unconjugated
• Format: Azide and BSA Free
• Concentration: 1 mg/ml

Scn1a Antibody (K74/71) - Chimeric - Azide and BSA Free Summary

• Description: This recombinant antibody is a chimeric antibody created by replacing the mouse heavy and light constant regions of clone K74/71 with chicken IgY heavy and light constant regions. As such this antibody retains the same binding performance as the original clone K74/71 but can be detected using standard anti-chicken secondary antibodies allowing flexibility for multiplexing applications. This antibody is expressed recombinantly in HEK293 cells and then affinity purified from the cell culture media and buffer exchanged into 1X PBS.
• Additional Information: Recombinant Monoclonal Antibody
• Immunogen: Fusion protein amino acids 1929-2009 (cytoplasmic C-terminus) of rat Scn1a (Uniprot#: P04774) produced recombinantly in E. Coli
• Specificity: No cross-reactivity with Nav1.2, Nav1.3 and Nav1.8
• Isotype: IgY
• Clonality: Monoclonal
• Host: Chicken
• Gene: SCN1A
• Purity: Affinity purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Immunocytochemistry/ Immunofluorescence
  • Immunohistochemistry-Paraffin
  • Immunoprecipitation
  • Western Blot 1:500
• Theoretical MW: 220 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: No Preservative
• Concentration: 1 mg/ml
• Purity: Affinity purified

Alternate Names for Scn1a Antibody (K74/71) - Chimeric - Azide and BSA Free

• brain I alpha subunit
• DEE6
• DEE6A
• DEE6B
• DRVT
• EIEE6
• FEB3
• FEB3A
• FHM3
• GEFSP2
• HBSCI
• NAC1
• Nav1.1
• SCN1
• SMEI
• sodium channel, voltage-gated, type I, alpha polypeptide
• sodium channel, voltage-gated, type I, alpha subunit

Background

Voltage-dependent sodium channels are heteromeric complexes that regulate sodium exchange between intracellular and extracellular spaces and are essential for the generation and propagation of action potentials in muscle cells and neurons. Each sodium channel is composed of a large pore-forming, glycosylated alpha subunit and two smaller beta subunits. This gene encodes a sodium channel alpha subunit, which has four homologous domains, each of which contains six transmembrane regions. Allelic variants of this gene are associated with generalized epilepsy with febrile seizures and epileptic encephalopathy. Alternative splicing results in multiple transcript variants. The RefSeq Project has decided to create four representative RefSeq records. Three of the transcript variants are supported by experimental evidence and the fourth contains alternate 5' untranslated exons, the exact combination of which have not been experimentally confirmed for the full-length transcript. [provided by RefSeq, Oct 2015]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: SCN1A