SC65 Antibody (1E12) - Azide and BSA Free

Western Blot: SC65 Antibody (1E12) [H00010609-M01] - Analysis of SC65 expression in transfected 293T cell line by SC65 monoclonal antibody (M01), clone 1E12.Lane 1: SC65 transfected lysate (Predicted MW: 50.381 KDa).Lane 2: Non-transfected lysate.

Western Blot: SC65 Antibody (1E12) [H00010609-M01] - SC65 monoclonal antibody (M01), clone 1E12. Western Blot analysis of SC65 expression in HepG2 ( Cat # L019V1 ).

• Reactivity: Hu
• Applications: WB, ELISA
• Clone: 1E12
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free

SC65 Antibody (1E12) - Azide and BSA Free Summary

• Immunogen: SC65 (NP_006446, 270 a.a. ~ 379 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. QCKVDCEANLTPNVGGYFVDKFVATMYHYLQFAYYKLNDVRQAARSAASYMLFDPKDSVMQQNLVYYRFHRARWGLEEEDFQPREEAMLYHNQTAELRELLEFTHMYLQS
• Specificity: SC65 - synaptonemal complex protein SC65 (1E12)
• Isotype: IgG2a Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: P3H4
• Purity: IgG purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Western Blot 1:500
• Application Notes: Antibody reactive against cell lysate and recombinant protein for western blot. It has also been used for ELISA.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: In 1x PBS, pH 7.4
• Preservative: No Preservative
• Purity: IgG purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for SC65 Antibody (1E12) - Azide and BSA Free

• leprecan-like 4
• Leprecan-like protein 4
• NO55, 55kDa
• NOL55
• Nucleolar autoantigen No55
• SC65rat synaptonemal complex protein
• synaptonemal complex protein SC65

Background

This nucleolar protein was first characterized because it was an autoantigen in cases on interstitial cystitis. The protein, with a predicted molecular weight of 50 kDa, appears to be localized in the particulate compartment of the interphase nucleolus, with a distribution distinct from that of nucleolar protein B23. During mitosis it is associated with chromosomes. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: P3H4
• Entrez:
  • 10609(Human)
• Uniprot:
  • NP_006446(Human)