SART3 Antibody

Western Blot: SART3 Antibody [NB100-1893] - (1ug/ml) analysis of Daudi lysate (RIPA buffer, 30ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.

• Reactivity: Hu
• Applications: WB, ELISA
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: 0.5 mg/ml

SART3 Antibody Summary

• Immunogen: Peptide with sequence C-MSNADFAKLFLRK corresponding to C-Terminus according to NP_055521.1.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: SART3
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Peptide ELISA Detection limit 1:16000
  • Western Blot 0.01 - 0.03 ug/ml
• Application Notes: WB: Approx. 170 kDa band observed in Daudi, Jurkat and MOLT4 lysates is reported in scientific literature (PMID: 10463607).
• Control:

  SART3 Overexpression Lysate

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for SART3 Antibody

• DSAP1
• hSART-3
• KIAA0156squamous cell carcinoma antigen recognised by T cells 3
• P100
• p110
• p110(nrb)
• RP11-13G14
• SART-3
• squamous cell carcinoma antigen recognized by T cells 3
• squamous cell carcinoma antigen recognized by T-cells 3
• Tat-interacting protein of 110 kDa
• Tip110
• TIP110MGC138188

Background

The protein encoded by this gene is an RNA-binding nuclear protein that is a tumor-rejection antigen. This antigen possesses tumor epitopes capable of inducing HLA-A24-restricted and tumor-specific cytotoxic T lymphocytes in cancer patients and may be useful for specific immunotherapy. This gene product is found to be an important cellular factor for HIV-1 gene expression and viral replication. It also associates transiently with U6 and U4/U6 snRNPs during the recycling phase of the spliceosome cycle. This encoded protein is thought to be involved in the regulation of mRNA splicing.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for SART3 Antibody (NB100-1893)(1)

Publication using NB100-1893

• Bell M, Schreiner S, Damianov A et al. p110, a novel human U6 snRNP protein and U4/U6 snRNP recycling factor. EMBO J 2002-06-03 [PMID: 12032085]

Bioinformatics

• Gene Symbol: SART3
• Entrez:
  • 9733(Human)
• Uniprot:
  • NP_001036.1.(Human)