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Recombinant Human SAGE1 Protein

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12.5% SDS-PAGE Stained with Coomassie Blue.
12.5% SDS-PAGE Stained with Coomassie Blue.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ELISA, PA, AP

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Recombinant Human SAGE1 Protein Summary

Description
A recombinant protein with a N-terminal GST tag corresponding to the amino acid sequence 357-455 of Human SAGE1

Source: Wheat Germ

Amino Acid Sequence: PLPSNALSTVLPGLAYLATADMPAMSTRDQHATIIHNLREEKKDNSQPTPDNVLSAVTPELINLAGAGIPPMSTRDQYATVNHHVHEARMENGQRKQDN
Details of Functionality
This protein is not active and should not be used for experiments requiring activity.
Source
Wheat germ
Protein/Peptide Type
Partial Recombinant Protein
Gene
SAGE1
Purity
>80% by SDS-PAGE and Coomassie blue staining

Applications/Dilutions

Application Notes
This protein has not been tested for any functionality. Product may contain endotoxins and is not suitable for use with live cells.
Theoretical MW
36.63 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -80C. Avoid freeze-thaw cycles.
Buffer
50 mM Tris-HCI, 10 mM reduced Glutathione, pH 8.0 in the elution buffer.
Purity
>80% by SDS-PAGE and Coomassie blue staining

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for Recombinant Human SAGE1 Protein

  • putative tumor antigen
  • SAGECT14Cancer/testis antigen 14
  • sarcoma antigen 1

Background

SAGE1 - sarcoma antigen 1

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

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[Unconjugated]
664-LI/CF
1 µg/lane of Recombinant Human LIGHT/TNFSF14 (Catalog # 664-LI/CF) was resolved with SDS-PAGE under reducing (R) conditions and visualized by silver staining, showing a band at 25 kDa.
Recombinant Human LIGHT/TNFSF14 (Catalog # 664-LI/CF) stimulates cell proliferation in HUVEC human umbilical vein endothelial cells. The ED50 is 1-4 ng/mL.
(10 Publications)
Species: Hu
Melanoma antigen
family C2 Antibody
NBP2-34145
Immunocytochemistry/Immunofluorescence: Melanoma antigen family C2 Antibody [NBP2-34145] - Staining of human cell line SK-MEL-30 shows localization to nucleus & nucleoli.
Immunohistochemistry-Paraffin: Melanoma antigen family C2 Antibody [NBP2-34145] - Staining in human testis and endometrium tissues using anti-MAGEC2 antibody. Corresponding MAGEC2 RNA-seq data are presented for the same tissues.
Immunohistochemistry-Paraffin: Melanoma antigen family C2 Antibody [NBP2-34145] - Staining of human endometrium shows low expression as expected.
Species: Hu
Applications: ICC/IF, IHC, IHC-P
c-Fos Antibody
(2H2)
NBP2-50037
Knockout Validated: c-Fos Antibody (2H2) [NBP2-50037] - Western blot shows lysates of HeLa human cervical epithelial carcinoma parental cell line and c-Fox knockout (KO) HeLa cell line. PVDF membrane was probed with 1:1000 of Mouse Anti-Human c-Fox Monoclonal Antibody (Catalog # NBP2-50037) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog #HAF018). Specific band was detected for c-Fox at approximately 52 kDa (as indicated) in the parental HeLa cell line, but is not detectable in the knockout HeLa cell line. This experiment was conducted under reducing conditions.
Western Blot: c-Fos Antibody (2H2) [NBP2-50037] - Top panel: Analysis of c-Fos expression in HeLa cells using NBP2-50037. Lane 1: HeLa cells were serum-starved for 36 hours. Lane 2: Serum-starved HeLa cells were stimulated with 20% FBS (fetal bovine serum) for 2 hours. NBP2-50037 recognizes bands in the range of 50-65 kDa, which represent multiple forms of c-Fos. Serum starvation attenuates c-Fos expression, while 20% FBS strongly stimulates c-Fos expression. Bottom panel: Blot was stripped and probed with monoclonal antibody against GAPDH (NB300-221) used as loading control.
Immunocytochemistry/Immunofluorescence: c-Fos Antibody (2H2) [NBP2-50037] - Section of rat hippocampus stained with mouse monoclonal antibody to c-FOS NBP2-50037 in red and counterstained with rabbit polyclonal antibody to FOX3/NeuN. DAPI reveals nuclei of neurons and glia in blue. The hippocampal neurons stain green for FOX3/NeuN and a few also are expressing c-FOS, and so appear orange. These cells were spontaneously active at the time the animal was sacrificed.
(1 Review)
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC, ICC, KO
CTAG1B Antibody
(OTI2B6)
NBP1-47714
Western Blot: CTAG1B Antibody (2B6) [NBP1-47714] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CTAG1B (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CTAG1B.
Immunocytochemistry/Immunofluorescence: CTAG1B Antibody (2B6) [NBP1-47714] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY CTAG1B.
Immunohistochemistry-Paraffin: CTAG1B Antibody (2B6) [NBP1-47714] - Staining of paraffin-embedded Carcinoma of thyroid tissue using anti-CTAG1Bmouse monoclonal antibody.
Species: Hu, Mk
Applications: WB, ICC/IF, IHC, IHC-P
MAGEC1 Antibody
NBP1-85397
Immunohistochemistry-Paraffin: MAGEC1 Antibody [NBP1-85397] - Staining of human testis shows high expression.
Immunohistochemistry-Paraffin: MAGEC1 Antibody [NBP1-85397] - Staining of human epididymis shows low expression as expected.
Immunohistochemistry-Paraffin: MAGEC1 Antibody [NBP1-85397] - Staining in human testis and epididymis tissues using anti-MAGEC1 antibody. Corresponding MAGEC1 RNA-seq data are presented for the same tissues.
Species: Hu
Applications: IHC, IHC-P
SLC22A2/OCT2
Antibody
NBP1-89417
Immunohistochemistry-Paraffin: SLC22A2/OCT2 Antibody [NBP1-89417] - Staining of human skin shows strong cytoplasmic positivity in basal layer of epidermal cells.
(2 Publications)
Species: Hu, Rt
Applications: WB, IHC, IHC-P, Single Cell Western
CT45A1 Antibody
NBP2-46702
Immunocytochemistry/Immunofluorescence: CT45A1 Antibody [NBP2-46702] - Staining of human cell line U-2 OS shows localization to nucleus and nucleoli. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: CT45A1 Antibody [NBP2-46702] - Staining in human testis and liver tissues. Corresponding CT45A1 RNA-seq data are presented for the same tissues.
Immunohistochemistry-Paraffin: CT45A1 Antibody [NBP2-46702] - Staining of human liver shows no positivity in hepatocytes as expected.
Species: Hu
Applications: ICC/IF, IHC, IHC-P
SSX2 Antibody
(OTI4D10)
NBP1-48008
Western Blot: SSX2 Antibody (4D10) [NBP1-48008] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY SSX2 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-SSX2.
Immunocytochemistry/Immunofluorescence: SSX2 Antibody (4D10) [NBP1-48008] Staining of COS7 cells transiently transfected by pCMV6-ENTRY SSX2.
Immunohistochemistry-Paraffin: SSX2 Antibody (OTI4D10) [NBP1-48008] - SSX2 Antibody (4D10) [NBP1-48008] - Staining of paraffin-embedded Kidney tissue using anti-SSX2mouse monoclonal antibody.
Species: Hu
Applications: WB, Flow, ICC/IF, IHC, IHC-P
AKR1C2 Antibody
(3C11)
H00001646-M03
Western Blot: AKR1C2 Antibody (3C11) [H00001646-M03] - Analysis of AKR1C2 expression in transfected 293T cell line by AKR1C2 monoclonal antibody (M03), clone 3C11. Lane 1: AKR1C2 transfected lysatE (36.7 KDa). Lane 2: Non-transfected lysate.
ELISA: AKR1C2 Antibody (3C11) [H00001646-M03] - Detection limit for recombinant GST tagged AKR1C2 is approximately 0.3ng/ml as a capture antibody.
(2 Publications)
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Applications: WB, ELISA
Recombinant Human
SPANXA1 Protein
H00030014-P01
12.5% SDS-PAGE Stained with Coomassie Blue.
Species: Hu
SP17 Antibody
NBP1-85409
Western Blot: SP17 Antibody [NBP1-85409] - Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10. Lane 2: Negative control (vector only transfected HEK293T lysate). Lane 3: Over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).
Immunocytochemistry/Immunofluorescence: SP17 Antibody [NBP1-85409] - Staining of human cell line U-2 OS shows localization to vesicles.
Immunohistochemistry-Paraffin: SP17 Antibody [NBP1-85409] - Staining in human testis and endometrium tissues using anti-SPA17 antibody. Corresponding SPA17 RNA-seq data are presented for the same tissues.
Species: Hu
Applications: WB, ICC/IF, IHC, IHC-P
SERCA2 ATPase
Antibody (2A7-A1)
NB300-581
Immunocytochemistry/Immunofluorescence: SERCA2 ATPase Antibody (2A7-A1) [NB300-581] - SERCA2 ATPase staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with or an antibody recognizing SERCA2 ATPase at a dilution of 1:100-1:200 over night at 4C, washed with PBS and incubated with a DyLight-488 conjugated.
Immunohistochemistry-Paraffin: SERCA2 ATPase Antibody (2A7-A1) [NB300-581] - Both normal and cancer biopsies of deparaffinized Human skeletal muscle tissues.
Immunocytochemistry/Immunofluorescence: SERCA2 ATPase Antibody (2A7-A1) [NB300-581] - SERCA2 ATPase staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with or an antibody recognizing SERCA2 ATPase at a dilution of 1:100-1:200 over night at 4C, washed with PBS and incubated with a DyLight-488 conjugated.
(11 Publications)
Species: Hu, Mu, Rt, Po, Am, Ca, GP, Rb, Sh
Applications: WB, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP, ICC, IF
FMN1 Antibody
44110002
ELISA: FMN1 Antibody [44110002]
(3 Publications)
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Applications: WB, ELISA, ICC/IF
Tau Antibody (2E9)
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Western Blot: Tau Antibody (2E9) [NBP2-25162] - Analysis of different tissue lysates using mouse mAb to MAP-tau, NBP2-25162, dilution 1:2,000 in green: [1] protein standard (red), [2] rat brain, [3] rat spinal cord, [4] mouse brain, [5] mouse spinal cord. Tau protein is expressed as up to 9 different isoforms of different molecular weight, and so appears as multiple closely spaced bands in the range from 48 kDa to 67 kDa in the CNS and including larger big tau forms in the PNS, visible in lane 5.
Immunocytochemistry/Immunofluorescence: Tau Antibody (2E9) [NBP2-25162] - Analysis of cortical neuron-glial culture from E20 rat stained with mouse mAb to MAP-tau, NBP2-25162, dilution 1:1,000 in green, and costained with chicken pAb to MAP2, dilution 1:5,000 in red. The blue is DAPI staining of nuclear DNA. NBP2-25162 antibody stains perikarya, dendrites and axons of neurons, while MAP2 antibody labels only dendrites and perikarya. As a result, perikarya and dendrites appear orange-yellow, since they contain both proteins.
Immunocytochemistry/Immunofluorescence: Tau Antibody (2E9) [NBP2-25162] - E18 primary rat hippocampal neurons (from Neuromics) were fixed and immunostained with NBP2-25162 (green), chicken antibody to alpha Internexin (NBP1-05208, red) and for DNA (blue). NBP2-25162 stains the neuronal perikarya and processes strongly, and does not stain non neuronal cells in these cultures. The alpha Internexin antibody stains intermediate or 10nm filament bundles in the cytoplasm of these cells.
Species: Hu, Mu, Rt, Po, Bv, Eq
Applications: WB, ICC/IF, IHC
FoxC2 Antibody
NB100-1269
Western Blot: FoxC2 Antibody [NB100-1269] - HEK293 lysate (10 ug protein in RIPA buffer) over expressing Human FOXC2 with DYKDDDDK tag probed with EB06007 in Lane A,and probed with anti- DYKDDDDK Tag in lane C. Mock-transfected HEK293 probed with EB06007 in Lane B. Primary incubations were for 1 hour. Detected by chemiluminescence.
Immunohistochemistry-Paraffin: FoxC2 Antibody [NB100-1269] - staining of paraffin embedded fetal renal tissue using anti- FoxC2 antibody. Image from verified customer review.
(2 Reviews)
(4 Publications)
Species: Hu, Mu
Applications: WB, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, PEP-ELISA
EGR1 Antibody
(6E8.)
H00001958-M03
Western Blot: EGR1 Antibody (6E8) [H00001958-M03] - Analysis of EGR1 expression in transfected 293T cell line by EGR1 monoclonal antibody (M03), clone 6E8. Lane 1: EGR1 transfected lysatE (59.73 KDa). Lane 2: Non-transfected lysate.
Immunocytochemistry/Immunofluorescence: EGR1 Antibody (6E8) [H00001958-M03] - Analysis of monoclonal antibody to EGR1 on HeLa cell. Antibody concentration 10 ug/ml
Immunohistochemistry-Paraffin: EGR1 Antibody (6E8) [H00001958-M03] - Analysis of monoclonal antibody to EGR1 on formalin-fixed paraffin-embedded human placenta. Antibody concentration 3 ug/ml
(2 Publications)
Species: Hu, Rb
Applications: WB, ELISA, ICC/IF, IHC-P
NXF2 Antibody
NBP2-15049
Western Blot: NXF2 Antibody [NBP2-15049] - Sample (30 ug of whole cell lysate) A: Jurkat 7.5% SDS PAGE; antibody diluted at 1:1000.
Immunocytochemistry/Immunofluorescence: NXF2 Antibody [NBP2-15049] - Paraformaldehyde-fixed A431, using antibody at 1:200 dilution.
Species: Hu
Applications: WB, ICC/IF
5-HT1A Antibody
NBP2-21590
Western Blot: 5-HT1A Antibody [NBP2-21590] - Sample (30 ug of whole cell lysate) A: Molt-4 B: Raji 10% SDS PAGE gel, diluted at 1:1000.
Immunohistochemistry-Paraffin: 5-HT1A Antibody [NBP2-21590] - Paraffin-embedded mouse duodenum. 5HT1A Receptor antibody [N3C1], Internal dilution: 1:500.
Western Blot: 5-HT1A Antibody [NBP2-21590] - Sample (30 ug of whole cell lysate) A:NIH-3T3 10% SDS PAGE gel, diluted at 1:1000.
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC, IHC-Fr, IHC-P
vH RAS Antibody
(OTI1D9)
NBP2-45745
Western Blot: vH RAS Antibody (1D9) [NBP2-45745] - Analysis of extracts (35ug) from 9 different cell lines (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).
Immunohistochemistry: vH RAS Antibody (1D9) [NBP2-45745] - Analysis of Adenocarcinoma of Human ovary tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)
Western Blot: vH RAS Antibody (1D9) [NBP2-45745] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY vH RAS.
Species: Hu
Applications: WB, IHC, IF
SLC22A3 Antibody
NBP1-83976
Immunocytochemistry/Immunofluorescence: SLC22A3 Antibody [NBP1-83976] - Immunofluorescent staining of human cell line A549 shows localization to cytosol & vesicles.
Immunohistochemistry-Paraffin: SLC22A3 Antibody [NBP1-83976] - Staining of human placenta shows strong cytoplasmic positivity in trophoblastic cells.
Species: Hu
Applications: ICC/IF, IHC, IHC-P

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Bioinformatics

Gene Symbol SAGE1