RYBP/DEDAF Antibody - BSA Free

Western Blot: RYBP/DEDAF Antibody [NBP1-76842] - Human A549 (lane A), HepG2 (lane B), and mouse 3T3 (lane C) cell lysates with RYBP/DEDAF antibody at 1 ug /ml.

Immunocytochemistry/ Immunofluorescence: RYBP/DEDAF Antibody - BSA Free [NBP1-76842] - Immunofluorescence of RYBP/DEDAF in mouse liver tissue with RYBP/DEDAF antibody at 20 ug/ml.

Immunohistochemistry: RYBP/DEDAF Antibody - BSA Free [NBP1-76842] - Immunohistochemistry of RYBP/DEDAF in mouse liver tissue with RYBP/DEDAF antibody at 10 ug/mL.

Immunocytochemistry/ Immunofluorescence: RYBP/DEDAF Antibody - BSA Free [NBP1-76842] - Immunofluorescence of RYBP/DEDAF in A549 cells with RYBP/DEDAF antibody at 20 ug/mL.

Immunohistochemistry: RYBP/DEDAF Antibody - BSA Free [NBP1-76842] - Immunohistochemistry of RYBP/DEDAF in mouse liver tissue with RYBP/DEDAF antibody at 5 ug/mL.

• Reactivity: Hu, Mu, Rt
• Applications: WB, ELISA, ICC/IF, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1 mg/ml

RYBP/DEDAF Antibody - BSA Free Summary

• Description: Novus Biologicals Rabbit RYBP/DEDAF Antibody - BSA Free (NBP1-76842) is a polyclonal antibody validated for use in IHC, WB, ELISA and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Antibody was raised against a 14 amino acid peptide near the carboxy terminus of human DEDAF. The immunogen is located within the last 50 amino acids of DEDAF. Amino Acid Squence: TPKGDMSAVNDESF
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: RYBP
• Purity: Peptide affinity purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:100-1:2000
  • Immunocytochemistry/ Immunofluorescence 20 ug/ml
  • Immunohistochemistry 5 ug/ml
  • Immunohistochemistry-Paraffin 5 ug/ml
  • Western Blot 1 ug/ml
• Theoretical MW: 32 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• Control Peptide:

  RYBP/DEDAF Peptide (NBP1-76842PEP)

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.02% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Peptide affinity purified

Alternate Names for RYBP/DEDAF Antibody - BSA Free

• AAP1
• APAP-1
• Apoptin-associating protein 1
• Death effector domain-associated factor
• DEDAFYY1 and E4TF1-associated factor 1
• RING1 and YY1 binding protein
• RING1 and YY1-binding protein
• ring1 interactor RYBP
• YEAF1DED-associated factor
• YY1 and E4TF1 associated factor 1

Background

Apoptosis is related to many diseases and induced by a family of cell death receptors and their ligands. Cell death signals are transduced by death domain (DD) death effector domain (DED), and caspase recruitment domain (CARD) containing molecules. Several molecules including caspases and adaptor FADD contain DEDs. A novel protein that interacts with DED of caspase-8 and 10, and FADD was identified recently and designated DEDAF for DED associated factor . DEDAF is identical to the transcriptional repressor RYBP. DEDAF/RYBP is expressed in multiple tissues and cell lines. DEDAF interacts with FADD and augments the formation of CD95/FADD/capase-8 complexes at the cell membrane, and interacts with DED-containing DNA biding protein (DEDD) in the nucleus indicating it is involved in the regulation of both cytoplasmic and nuclear events of apoptosis.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: RYBP
• Entrez:
  • 23429(Human)
  • 56353(Mouse)
• Uniprot:
  • Q8N488(Human)