Rat Ryanodine Receptor 1 ELISA Kit (Colorimetric)

• Reactivity: Rt
• Applications: ELISA
• Suitable Sample Type: Tissue homogenates, Cell lysates and other biological fluids
• Standard Curve Range: 0.625 - 40 ng/mL (example only; lot dependent)
• Sensitivity: 0.231 ng/mL (example only; lot dependent)

Rat Ryanodine Receptor 1 ELISA Kit (Colorimetric) Summary

• Description: Assay time: 3 hours
• Standard Curve Range: 0.625 - 40 ng/mL (example only; lot dependent)
• Sensitivity: 0.231 ng/mL (example only; lot dependent)
• Assay Type: Sandwich ELISA
• Inter-Assay: %CV < 12 (example only; lot dependent)
• Intra-Assay: %CV < 10 (example only; lot dependent)
• Sample Volume: 100 uL
• Kit Type: ELISA Kit (Colorimetric)
• Gene: RYR1

Applications/Dilutions

• Dilutions:
  • ELISA

Packaging, Storage & Formulations

• Storage: Storage of components varies. See protocol for specific instructions.

Alternate Names for Rat Ryanodine Receptor 1 ELISA Kit (Colorimetric)

• CCO
• central core disease of muscle
• MHS
• MHS1
• PPP1R137
• protein phosphatase 1, regulatory subunit 137
• ryanodine receptor 1 (skeletal)
• Ryanodine Receptor 1
• ryanodine receptor type1
• RYDR
• RYR
• RyR1
• RYR-1
• sarcoplasmic reticulum calcium release channel
• Skeletal muscle calcium release channel
• skeletal muscle ryanodine receptor
• Skeletal muscle-type ryanodine receptor
• SKRR
• type 1-like ryanodine receptor

Background

The Ryanodine Receptor (RyR) is the channel responsible for the release of calcium from the sarcoplasmic reticulum (SR) in muscle cells and also plays a role in calcium regulation in non-muscle cells. The RyR exists as a homotetramer and is predicted to have a short cytoplasmic C-terminus and 4-10 transmembrane domains; the remainder of the protein, termed the "foot" region is located in the cytoplasm between the T-tubule and the SR. The mammalian RyR is the product of three different genes: RyR 1, which is expressed predominantly in skeletal muscle and areas of the brain, RyR 2, which is expressed predominantly in the heart muscle but also found in the stomach, endothelial cells and diffuse areas of the brain, and RyR-3 which is found in smooth muscle and the brain (striatum, thalamus and hippocampus).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

FAQs for Ryanodine Receptor 1 ELISA Kit (NBP3-42816). (Showing 1 - 1 of 1 FAQ).

1. What is the difference between the Quantikine and Duoset ELISA kits?
   • Our Quantikine kits are fully optimized and validated for the sample types listed on the product specific webpage and datasheet, as this can vary between kits. Each kit is supplied ready to use with one pre-coated 96-well plate, a detection antibody directly conjugated to HRP, and all other necessary reagents. These kits are ideal for researchers who want the convenience of a ready to use and optimized ELISA product. Some of these kits are also available prepackaged in larger 6 and 50 plate sizes.Our DuoSet Kits, in contrast, are ELISA development kits containing the capture and detection antibody, the mass-value calibrated standard, and streptavidin-HRP to prepare approximately 5 or 15 plates. Ancillary reagents will need to be used/purchased, and for most kits, we will recommend one of our Ancillary Reagent Kits, which contain the reagents we use ourselves in-house. DuoSet kits are validated only for cell culture supernatant samples and therefore require further development and validation for accurate measurement in more complex samples such as serum and plasma. Our DuoSet Kits offer an economical, flexible alternative for the experienced ELISA user.

Bioinformatics

• Gene Symbol: RYR1